Mohammadreza Khoshayand

[ 1 ] - Characterization and Classification of Iranian Honey Based on Physicochemical Properties and Antioxidant Activities, with Chemometrics Approach

In the present study, the physicochemical properties and antioxidant activities of different Iranian honey samples are investigated using various multivariate techniques in order to develop a quality control model. Forty-eight Iranian honey samples were tested for 15 physicochemical and antioxidant parameters. The parameters for which the samples were tested included color intensity, moisture, ...

[ 2 ] - Screening for Type II L-Asparaginases: Lessons from the Genus Halomonas

Among the two types of bacterial L-asparaginases, only type II enzymes have been used inthe treatment of acute lymphoblastic leukemia owing to their higher affinity for L-asparagine.However, current screening media used for the isolation of L-asparaginase-producingmicroorganisms do not discriminate between the two types of L-asparaginase. During anoptimization study conducted to increase L-aspa...

[ 3 ] - Nonionic Surfactants (Dodecyl Maltoside and Polysorbate 20) Effect on Light induced Aggregation and Conformational Changes of Recombinant Human IFNβ_1b

Liquid protein formulations are prone to form aggregates. The effect of nonionic surfactants such as Polysorbate 20 (PS 20) and n-Dodecyl β-D-maltoside (DDM) on the prevention of aggregation and conformational changes of recombinant human IFNβ-1b (rhIFN β_1b) was explored. Polysorbate has been used in formulations of protein pharmaceuticals. There have been concerns about using PS 20 due to its...

[ 4 ] - Blood concentrations of cadmium and lead in multiple sclerosis patients from Iran

Since industrial revolution heavy metals such as lead (Pb) and cadmium (Cd) have been extensively dispersed in environment which, unknown biological effects and prolong biological half-life make them as a major hazard to human health. In addition, the sharp increase in Multiple sclerosis incidence rates has been recorded in Iran. The propose of this study was to measuring blood lead and cadmium...

[ 5 ] - L-Asparaginase Activity in Cell Lysates and Culture Media of Halophilic Bacterial Isolates

The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated the ability to gro...

[ 6 ] - Docetaxel loaded PEG-PLGA nanoparticles: optimized drug loading, in vitro cytotoxicity and in vivo antitumor effect

In this study a 3-factor, 3-level Box-Behnken design was used to prepare optimized docetaxel (DTX) loaded pegylated poly lactide-co-glycolide (PEG-PLGA) NPs with polymer concentration (X1), drug concentration (X2) and ratio of the organic to aqueous solvent (X3) as the independent variables and particle size (Y1), poly dispersity index (PDI) (Y2) and drug loading (Y3) as the responses. The cyto...

[ 7 ] - Characterization and Classification of Iranian Honey Based on Physicochemical Properties and Antioxidant Activities, with Chemometrics Approach

In the present study, the physicochemical properties and antioxidant activities of different Iranian honey samples are investigated using various multivariate techniques in order to develop a quality control model. Forty-eight Iranian honey samples were tested for 15 physicochemical and antioxidant parameters. The parameters for which the samples were tested included color intensity, moisture, ...

[ 8 ] - Screening for Type II L-Asparaginases: Lessons from the Genus Halomonas

Among the two types of bacterial L-asparaginases, only type II enzymes have been used inthe treatment of acute lymphoblastic leukemia owing to their higher affinity for L-asparagine.However, current screening media used for the isolation of L-asparaginase-producingmicroorganisms do not discriminate between the two types of L-asparaginase. During anoptimization study conducted to increase L-aspa...

[ 9 ] - Nonionic Surfactants (Dodecyl Maltoside and Polysorbate 20) Effect on Light induced Aggregation and Conformational Changes of Recombinant Human IFNβ_1b

Liquid protein formulations are prone to form aggregates. The effect of nonionic surfactants such as Polysorbate 20 (PS 20) and n-Dodecyl β-D-maltoside (DDM) on the prevention of aggregation and conformational changes of recombinant human IFNβ-1b (rhIFN β_1b) was explored. Polysorbate has been used in formulations of protein pharmaceuticals. There have been concerns about using PS 20 due to its...

[ 10 ] - Blood concentrations of cadmium and lead in multiple sclerosis patients from Iran

Since industrial revolution heavy metals such as lead (Pb) and cadmium (Cd) have been extensively dispersed in environment which, unknown biological effects and prolong biological half-life make them as a major hazard to human health. In addition, the sharp increase in Multiple sclerosis incidence rates has been recorded in Iran. The propose of this study was to measuring blood lead and cadmium...

[ 11 ] - L-Asparaginase Activity in Cell Lysates and Culture Media of Halophilic Bacterial Isolates

The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated the ability to gro...

[ 12 ] - Docetaxel loaded PEG-PLGA nanoparticles: optimized drug loading, in vitro cytotoxicity and in vivo antitumor effect

In this study a 3-factor, 3-level Box-Behnken design was used to prepare optimized docetaxel (DTX) loaded pegylated poly lactide-co-glycolide (PEG-PLGA) NPs with polymer concentration (X1), drug concentration (X2) and ratio of the organic to aqueous solvent (X3) as the independent variables and particle size (Y1), poly dispersity index (PDI) (Y2) and drug loading (Y3) as the responses. The cyto...

[ 13 ] - Effect of powder sizes, pH of water, ultrasound and method of distillation on extraction of fennel essential oil

The essential oils are frequently used in food processing industry, perfumery, cosmetics and pharmaceuticals. Most of the oils obtained by steam distillation and water distillation. It is necessary to specify the best conditions of essential oils production to get much more major compound(s) and higher yield oil. So, fennel oil was selected in this study. The objective of this work was to ident...

[ 14 ] - Enhanced Production and Characterization of a Highly Stable Extracellular Protease from an Extreme Halophilic Isolate Salicola marasensis

Owing to their superior catalytic activity in the extreme conditions, extremozymes have found the potential biotechnological applications for industrial purposes. A robust extracellular protease activity was detected in the culture broth of Salicola marasensis, an extreme halophilic bacterium, after a 48 h-incubation. The effect of different media ingredients in a liquid state fermentation was ...

[ 15 ] - Determination of Biological Activity of Recombinant Reteplase Using Clot Lysis Time and Activated Partial Thromboplastin Time (APTT) Lysis Methods: A Comparative Study

Recombinant plasminogen activator (reteplase) is a third generation thrombolytic agent which has been used on coronary artery thrombosis and acute myocardial infarction. Clot lysis assay is usually considered as a unique method to evaluate biological activity of reteplase. In this study biological activity of reteplase was determined by APTT (activated partial thromboplastin time) lysis method....

[ 16 ] - Fingolimod SLNs: Preparation, in vitro evaluation and Optimization of lyophilization using D-Optimal Experimental Design

Abstract Multiple Sclerosis (MS) is one of the most common neurological disorders diagnosed in young adults. there are no current cures for the disease or its underlying causes, some drugs have been developed that can decrease or delay disease progression. Fingolimod is an immunomodulating drug, mostly used for treating multiple sclerosis (MS). It approximately halves the rate of relapse ...

[ 17 ] - Enhanced Production and Characterization of a Highly Stable Extracellular Protease from an Extreme Halophilic Isolate Salicola marasensis

Owing to their superior catalytic activity in the extreme conditions, extremozymes have found the potential biotechnological applications for industrial purposes. A robust extracellular protease activity was detected in the culture broth of Salicola marasensis, an extreme halophilic bacterium, after a 48 h-incubation. The effect of different media ingredients in a liquid state fermentation was ...

[ 18 ] - Determination of Biological Activity of Recombinant Reteplase Using Clot Lysis Time and Activated Partial Thromboplastin Time (APTT) Lysis Methods: A Comparative Study

Recombinant plasminogen activator (reteplase) is a third generation thrombolytic agent which has been used on coronary artery thrombosis and acute myocardial infarction. Clot lysis assay is usually considered as a unique method to evaluate biological activity of reteplase. In this study biological activity of reteplase was determined by APTT (activated partial thromboplastin time) lysis method....