Mehrdad Gholami

Dept. of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran | Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran

[ 1 ] - Frequency of 16S rRNA Methylase and Aminoglycoside-Modifying Enzyme Genes among Clinical Isolates of Acinetobacter baumannii in Iran

Background & objective: Multidrug-resistant Acinetobacter baumannii (MDR-AB) is an important nosocomial pathogen which is associated with significant morbidity and mortality, particularly in high-risk populations. Aminoglycoside-modifying enzymes (AMEs) and 16S ribosomal RNA (16S rRNA) methylation are two important mechanisms of resistance to ...

[ 2 ] - Determination of Helicobacter pylori virulence-associated genes in duodenal ulcer and gastric biopsies

Background: Helicobacter pylori (H. pylori or Hp) has been strongly associated with the peptic ulcer diseases, chronic gastritis, ulcers, and gastric cancer. Genes associated with pathogenicity have been designated for H. pylori, and some of them appear to be related to more severe clinical consequences of the infection. The present study was conducted to determine cagA, vacA, cagE, iceA1, oipA...

[ 3 ] - In silico Analysis and Molecular Modeling of RNA Polymerase, Sigma S (RpoS) Protein in Pseudomonas aeruginosa PAO1

Background: Sigma factors are proteins that regulate transcription in bacteria. Sigma factors can be activated in response to different environmental conditions. The rpoS (RNA polymerase, sigma S) gene encodes sigma-38 (σ38, or RpoS), a 37.8 kDa protein in Pseudomonas aeruginosa (P. aeruginosa) strains. RpoS is a central regulator of the general stress response and operates in both retroa...

[ 4 ] - Evaluation of Cell Growth Inhibition of Bifidobacterium Bifidum Cell-free Supernatant Extract on 4T1Tumor Cell Lineage

Background: Cancer is amongst the leading reasons of death in all parts of the world. Breast cancer is also responsible for the largest number of deaths among women population. Several studies confirmed that Bifidobacterium bifidum as a probiotic meaningfully inhibited breast cancer development. The present study aimed to investigate the effect of B. bifidum supernatant on the cell growth inhib...

[ 5 ] - Prevalence of MDR, XDR and PDR Phenotypes among Pseudomonas aeruginosa Strains Isolated from Hospitalized Patients in Mazandaran Province, Iran

Background and purpose: Pseudomonas aeruginosa is an opportunistic pathogen and the second leading cause of nosocomial infections, especially in the intensive care unit (ICU), and is responsible for high mortality rates. The aim of this study was to determine the pattern of antibiotic susceptibility and prevalence of multiple drug resistance strains of P. aeruginosa isolated from hospitalized p...

[ 6 ] - Molecular Identification of ESBLs Genes (blaTEM, blaSHV and blaCTX) among Non-fermenting Gram-negative Bacteria Isolated from Hospitalized Patients in Mazandaran Province, Iran

Background and purpose: Production of beta-lactamase enzymes by bacteria, especially Extended-spectrum β-lactamases (ESBLs) is one of the major problems worldwide. ESBLs are mostly located on bacterial plasmids and it is recognized that SHV, TEM, and CTX-M are prevailing among them. The aim of this study was to determine the ability to produce ESBLs genes based on molecular method among non-fer...