Mohammad Hasan Sheikhha
Genetic Department, Clinical and Research Center for Infertility, Shahid Sadughi University of Medical Sciences, Yazd, Iran
[ 1 ] - Glutathione S-transferases Null Genotype in Acute Myeloid Leukaemia
Background: The glutathione S-transferase (GST) family of metabolising enzymes plays an important role in the detoxification of mutagens and carcinogens. The expression of many of these cancer susceptibility enzymes is genetically polymorphic. An increased frequency of GST-null genotypes has been associated with several malignancies. Objective: To investigate the rate of GSTT1 and GSTM1 null ge...
[ 2 ] - بررسی ارتباط بین پلی‌مورفیسم GSTP1 Ile105Val و خطر اندومتریوز در یک جمعیت ایرانی
Introdution: Endometriosis is one of the most common gynecologic disorders and shows significantly elevated prevalence in industrial regions. Additionally, a possible genetic predisposition is assumed for the disease. Glutathione S-transferases (GSTs) are enzymes participated in the metabolism of many human disease-causing mutagens, carcinogens and environmental pollutants. A functionally signi...
[ 3 ] - جذب سلولی miRNA-101 و ارزیابی تأثیر آن بر القای سمیت سلولی و بیانیوبی کوئیتین لیگاز HECTH9 در سلولهای لوسمی میلوئیدی حاد (AML)
Introdution: In the present study, Lipofactamine 2000 was used as a cationic liposome for miR-101 transfection in order to investigate its cytotoxicity and its effect on the expression of ubiquitin ligase HECTH9 in acute myeloid leukemia cells (AML). Methods: MiR- 101 was transferred to KG-1 cells (myeloid cells) and HBMF-SPH (healthy bone marrow cells) using lipofectamine 2000 as a nano carri...
[ 4 ] - ارزیابی و مقایسه خواص فیزیکوشیمیایی، سمیت سلولی و توانایی بارگذاری miRNA درلیپوزوم‌‌های کاتیونی متفاوت به منظور کاربرد در ژن‌درمانی
Introduction: In the present study, various formulations of cationic liposomes were designed and prepared using different cationic lipids. It was performed to assess the physicochemical properties, miRNA loading ability and cellular toxicity rates of liposomes in order to use in gene therapy. Methods: Different cationic liposome formulations (F1-F4) containing various cationic lipids, DOTAP, D...
[ 5 ] - ارائه رویکردی نوین جهت سنتز، درون‌گیری داروهای آب دوست و ارزیابی فیزیکوشیمیایی نانوسامانه لیپوزومی حساس به pH؛ بهبود کارایی درمانی داروی دوکسوربیسین با هدف اثر گذاری بر درمان سرطان استخوان و کاهش اثرات جانبی دوکسوربیسین
Introduction: Cancer is one of the most harmful disease throughout the world. Doxorubicin is an anti-cancer agent, used in the treatment of various types of the cancer such as bone cancer. There are several adverse effects related to clinical usage of Doxorubicin for long time. The present study aimed to investigate the reducing side effects and enhancing the therapeutic effect by liposomal car...
[ 6 ] - تأثیر ناهنجاری‌های ژنتیکی در حکم سقط جنین در فقه امامیه
جنین انسان، موجود محترمی است که از بین بردن آن به حکم اولی و با استناد به ادلۀ اربعه جایز نخواهد بود. در این پژوهش که با روش تحلیلی ـ توصیفی صورت گرفته است، این مسئله بررسی می‌شود که آیا با تحقّق حالت‌هایی چون وجود ناهنجاری‌های ژنتیکی در جنین به‌گونه‌ای که از منظر پزشکی، امکان حیات او وجود نداشته باشد و خطر جانی هم مادر را تهدید کند، سقط جنین جایز خواهد بود؟ نتیجه‌ای که از پژوهش حاصل شد این است ...
[ 7 ] - مشروعیت انتخاب جنسیت از طریق PGD (تشخیص ژنتیکی پیش از لانه‌گزینی)
تشخیص ژنتیکی پیش از لانه‌گزینی حاصل تلفیق لقاح آزمایشگاهی (IVF) و تشخیص پیش از تولد (PND) است. در این روش، تشخیص بیماریهای ژنتیکی در رویان انسان، پیش از لانه‌گزینی، به والدین شانس شروع بارداری با علم به سالم بودن کودک و فقدان ناهنجاری‌های ژنتیکی را میدهد. تعیین جنسیت فرزندان یکی از کاربردهای مهم تشخیص ژنتیکی پیش از لانه‌گزینی است که با دو هدف پیشگیری از بیماریهای وابسته به جنس و علاقۀ والد...
[ 8 ] - Investigating the rate of glutathione S-transferase T1 and M1 genes deletion in patients with lung cancer
Introduction: Glutathione S-tarnsferases (GST) gene family is one of the enzymes which is responsible for detoxifying mutagens and Carcinogens chemicals. People with null genotype of GSTM1 and GSTT1 genes are at higher risk of developing cancer, especially those who are related to smoking. The goal of this study was investigating the rate of glutathione S-transferase T1 and M1 genes deletion an...
[ 9 ] - ارزیابی اثراتmiR-143 لیپوزومه بر زیست پذیری سلولی و بیان ژن آنتی آپوپتوتیک Bcl-2 در سلول های استئوسارکومای انسانی جهت اثر گذاری بردرمان سرطان های متاستاتیک
Introdution: The present study was conducted to evaluate the effect of miR-143 on the expression of Bcl2 gene at the level of mRNA, cell cytotoxicity in the SaOs-2 cell line (Osteosarcoma) and Hum 63 (Human primary bone cells). Methods: In this experimental study, miR-143 was introduced into the SaOs-2 and Hum 63 cell by the lipofectam 2000 system. Then, the effects of miR-143 on cytotoxicity...
[ 10 ] - Study of anti-cancer effects of Curcumin; formulation of Curcumin-loaded nano carrier and its toxicity effect on MCF-7 Cell line.
Introdution: Nanotechnology introduced new methods to chemotherapy drugs delivery into cancer cells to reduce the side effect of drugs it increases the quality of cancer treatment and reduces the side effects of chemotherapy. . In this study, different lipid formulations of nucliposomes containing curcumin was prepared by thin-layer method and evaluated for chemical-physical evaluation on MCF-7...
[ 11 ] - Tumor Necrosis Factor Alpha -308 G/A Single Nucleotide Polymorphism and Risk of Sperm Abnormalities in Iranian Males
Objective Signaling molecules such as cytokines regulate spermatogenesis during the maturation of germ cells and apoptosis. Tumor necrosis factor-α (TNF-α) is one of the most-documented cytokines that supports spermatogenesis. We investigated the association of TNFα -308 Single Nucleotide Polymorphism with sperm abnormalities in Iranian population. MaterialsAndMethods This case control study in...
[ 12 ] - Angiotensin-Converting Enzymes, the Key Components in the Pathogenesis of COVID-19 Infection and their Role in the Interaction of SARS-Cov-2 with Human Host Cells
Introduction: Over the past 20 years, seven coronaviruses have caused more or less severe respiratory diseases in humans. Among these, the most important ones are SARS-CoV and the coronavirus, a similar virus that has created a pandemic called Covid-19 since 2019, belonging to the b-category of beta-coronaviruses called Sarbecovirus. This virus is due to a kind of spike-like structure to the A...
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