Fereshteh Eftekhar
Microbiology Department, Faculty of Biological Sciences, Shahid Beheshti University, G.C., Evin, Tehran, Iran
[ 1 ] - Biofilm Formation and Detection of IcaAB Genes in Clinical Isolates of Methicillin Resistant Staphylococcus aureus
Objective(s) Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial and community infections. Biofilm formation, mediated by a polysaccharide intercellular adhesin (PIA) and encoded by the ica operon, is considered to be an important virulence factor in both S. epidermidis and S. aureus. However, the clinical impact of the ica locus and PIA production is less w...
[ 2 ] - Genetic Profiling of Pseudomonas aeruginosa Isolates from Iranian Patients with Cystic Fibrosis Using RAPD-PCR and PFGE
Objective(s) Pseudomonas aeruginosa is the most important cause of chronic lung infections and death in patients with cystic fibrosis. Determining the distribution of specific strains within patient populations is important in order to examine the epidemiology of the disease and the possibility of cross infection among patients. Materials and Methods Forty six Iranian patients with cystic fib...
[ 3 ] - Antibacterial Activity of Twenty Iranian Plant Extracts Against Clinical Isolates of Helicobacter pylori
Objective(s) Due to increasing emergence of drug-resistance in Helicobacter pylori isolates, traditional plants are potentially valuable sources of novel anti-H. pylori agents. In this research, anti-H. pylori activity of the organic extracts of twenty native Iranian plants was determined against ten clinical isolates of H. pylori. Materials and Methods Disc diffusion was used to determine th...
[ 4 ] - ارتباط فنوتیپ آنزیم‌های بتالاکتاماز وسیع الطیف (ESBL) با حضور ژن بتالاکتامازهای blaTEM، blaSHV و blaCTX در جدایه های ادراری Klebsiella pneumoniae در تهران
جدایههای Klebsiella pneumoniae پاتوژنهای فرصت طلبی هستند که می توانند عامل مهمی در ایجاد عفونتهای جامعه و عفونتهای بیمارستانی باشند. برای درمان این عفونتها اغلب از آنتیبیوتیکهای بتالاکتام استفاده می شود و با افزایش مقاومت نسبت به این آنتیبیوتیکها، عمدتا با ایجاد بتالاکتامازهای وسیع الطیف توسط باکتری، درمان عفونتهای حاصل از این میکروارگانیسم با مشکل روبرو شده است. در این پژوهش، 196 ج...
[ 5 ] - Detection of Ampc and Extended-Spectrum Beta-Lactamases in Clinical Isolates of Pseudomonas Aeruginosa from Patients with Cystic Fibrosis
ABSTRACT Background and Objectives: Pseudomonas aeruginosa is the most frequent opportunistic pathogen isolated from the sputum of patients with cystic fibrosis (CF). Resistance to β -lactam antibiotics may arise from over expression of the naturally occurring AmpC cephalosporinases or acquired extended-spectrum β-lactamases (ESBL). The aim of...
[ 6 ] - آنالیز بیوانفورماتیکی پروتئین مایکوباکتریایی Rv1733c بعنوان کاندیدای واکسن، همسانه سازی و بیان آن در Escherichia coli
از آنجا که تعداد زیادی از جمعیت جهان به صورت نهفته با باسیل سل (Mycobacterium tuberculosis) آلوده هستند، واکسن هایی که سل نهفته را مورد هدف قرار بدهند تاثیر قابل توجهی در کنترل شیوع جهانی سل خواهند داشت. در این پژوهش ابتدا آنالیزهای بیوانفورماتیک روی یک پروتئین مایکوباکتریایی مربوط به فاز نهفته عفونت به نام Rv1733c انجام شد و پیش بینی شد که Rv1733c یک پروتئین غشایی با تعداد زیادی اپیتوپ های سلو...
[ 7 ] - Prevalence of qnr and aac(6’)-Ib-cr Genes in Clinical Isolates of Klebsiella Pneumoniae from Imam Hussein Hospital in Tehran
Background: Plasmid mediated quinolone resistance (PMQR) has been shown to play an important role in resistance not only to quinolones, but also β-lactams and aminoglycosides. In fact, qnr genes are frequently carried along with β-lactamase determinants on the same plasmids. We studied the prevalence of qnrA, qnrB, qnrS and aac(6’)-Ib-cr genes among quinolone and cephalosporin resistant clinica...
[ 8 ] - Correlation of Minimum Inhibitory Concentration Breakpoints and Methicillin Resistance Gene Carriage in Clinical Isolates of Staphylococcus epidermidis
Staphylococcus epidermidis is the most important member of coagulase negative staphylococci responsible for community and hospital acquired infections. Most clinical isolates of S. epidermidis are resistant to methicillin making these infections difficult to treat. In this study, correlation of methicillin resistance phenotype was compared with methicillin resistance (mecA) gene carriage in 55 ...
[ 9 ] - Correlation of quinolone-resistance, qnr genes and integron carriage in multidrug-resistant community isolates of Klebsiella spp.
Objective(s): Plasmid-mediated quinolone resistance (PMQR) determinants and integrons have a considerable contribution to bacterial drug resistance in Gram-negative pathogens. We studied the prevalence of PMQR genes and integron carriage in multidrug-resistant community isolates of Klebsiella spp.Materials and Methods: Two hundred and fi...
[ 10 ] - Prevalence of Extended-Spectrum β-lactamase and Integron Gene Carriage in Multidrug-Resistant Klebsiella Species Isolated from Outpatients in Yazd, Iran
Background: Community-acquired infections by multidrug-resistant (MDR), extended-spectrum β-lactamase (ESBL) producing Klebsiella species (Klebsiella spp.) is of major concern worldwide. Antibiotic resistance, production of extended-spectrum β-lactamases (ESBLs), and carbapenemases, as well as the presence of classes 1, 2, and 3 integrons in outpatient isolates of Klebsiella collected from Yazd...
[ 11 ] - Extraction of Outer membrane Vesicles from Vaccinal Strain of Bordetella Pertussis as the First Step of a Vaccine Candidate Study Against Pertussis Infection
Background: Pertussis is still one of the major public health problems. The increase of the disease emerged in recent decades due to the replacement of the reactogenic whole cell vaccine with the safer acellular vaccine and the genetic diversity of the bacterium. As outer membrane vesicles (OMVs) obtained from Bordetella pertussis contains surface immunogenic antigen in its structure, it has an...