Mohammad Reza Akbari Eydgahi

[ 1 ] - CFP10: mFcγ2 as a novel tuberculosis vaccine candidate increases immune response in mouse

Objective(s): Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-10 from Mycobacterium tuberculosis and the Fc-...

[ 2 ] - ساخت وکتور بیانی pcDNA3.1-FSHβ به منظور انتقال ژن زیر‌واحد FSHβ به سلول لاین پستان‌داران

سابقه و هدف: هورمون محرک فولیکولی (Follicle stimulating hormone, FSH)، یکی از هورمون‌های گلیکوپروتئینی هیپوفیز است که از دو زیر‌واحد آلفا و بتا تشکیل شده است. زیرواحد بتا حاوی 111 اسیدآمینه و یک سیگنال پپتید 18 اسیدآمینه‌ای و مسئول فعالیت بیولوژیکی هورمون می‌باشد. هدف از این مطالعه ساخت وکتور بیانی pcDNA3.1-FSHβ به منظور انتقال ژن زیر‌واحد FSHβ به سلول لاین پستانداران بود. مواد و روش‌...

[ 3 ] - Optimization and High Level Production of Recombinant Synthetic Streptokinase in E. coli Using Response Surface Methodology

Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...

[ 4 ] - Optimization and High Level Production of Recombinant Synthetic Streptokinase in E. coli Using Response Surface Methodology

Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...

[ 5 ] - Novel Small Molecules against Two Binding Sites of Wnt2 Protein as potential Drug Candidates for Colorectal Cancer: A Structure Based Virtual Screening Approach

Wnts are the major ligands responsible for activating Wnt signaling pathway through binding to Frizzled proteins (Fzd) as the receptors. Among these ligands, Wnt2 plays the main role in the tumorigenesis of several human cancers especially colorectal cancer (CRC). Therefore, it can be considered as a potential drug target.The aim of this study was to identify potential drug candidates ...

[ 6 ] - Novel Small Molecules against Two Binding Sites of Wnt2 Protein as potential Drug Candidates for Colorectal Cancer: A Structure Based Virtual Screening Approach

Wnts are the major ligands responsible for activating Wnt signaling pathway through binding to Frizzled proteins (Fzd) as the receptors. Among these ligands, Wnt2 plays the main role in the tumorigenesis of several human cancers especially colorectal cancer (CRC). Therefore, it can be considered as a potential drug target.The aim of this study was to identify potential drug candidates ...