Mehdi Imani
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran|Department of Cellular and Molecular Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran
[ 1 ] - Recombinant production and affinity purification of the FraC pore forming toxin using hexa-His tag and pET expression cassette
Objective(s): A newly-introduced protein toxin from a sea anemone, namely fragaceatoxin C is a protein with molecular weight of 20 kDa and pore-forming capability against cell membranes has recently grasped great attentions for its function. In this study, its coding sequence cloned as a fusion protein with His-tag for simple production and rapid purification. Materials and Methods: After PCR a...
[ 2 ] - Evidence of morphine like substance and μ-opioid receptor expression in Toxacara canis (Nematoda: Ascaridae)
Toxocara canis (Nematoda: Ascaridae) is an intestinal nematode parasite of dogs, which can also cause disease in humans. Transmission to humans usually occurs because of direct contact with T. canis eggs present in soil contaminated with the feces of infected dogs. This nematode has extraordinary abilities to survive for many years in different tissues of vertebrates, and deve...
[ 3 ] - مهندسی آنزیم درمانی اوریکاز آسپرژیلوس فلاووس با استفاده از روش جهش‌زایی هدف‌دار
Background & Aims: As a therapeutic enzyme, Aspergillus flavus (uricase or; EC 1.7.3.3), is used for treatment of urate deposits, gout and nephropathy, hyperuricemia and tumor lysis syndrom (TLS). Despite desirable kinetic features, fragile stability of uricase limits its wide range applications. Therefore, several approaches have developed such as protein engineering and genetic manipulations ...
[ 4 ] - اثر محافظتی سلول‌های مزانشیمی‌مغزاستخوان بر ویژگی‌های بافت شناسی و ریزسنجی بافت بیضه متعاقب پیچش-واپیچش بند بیضه در موش‌های سفید آزمایشگاهی
زمینه و هدف: با توجه به این که اختلال در سیستم گردش خون بیضه و متعاقباً کم خونی عضو میتواند موجب اختلال در باروری گردد. در این پژوهش اثر محافظتی سلول‌های مزانشیمی‌مغزاستخوان بر هیستومورفومتری بافت بیضه متعاقب پیچش ـ واپیچش Tursion-Detursion (T-D) بند بیضه به طور مقایسه‌ای مورد بررسی قرار گرفت. روش بررسی: در این مطالعه تجربی تعداد 30 سر موش سفید کوچک آزمایشگاهی بالغ به طور تصادفی به سه گروه م...
[ 5 ] - Investigation of the effect of experimental polycystic ovarian syndrome induced by stradiolvalerate on oocyte quality and in vitro fertilization potential and evaluation of vitamin E supplementation to emryo culture in mouse model
Background & Aims: One of the most common causes of infertility in women is ovarian causes, especially polycystic ovary syndrome (PCOS). However, in patient with PCOS, the number of oocytes taken increased during the IVF, but these oocytes have often low quality, which ultimately leads to reduce fertilization potential. The aim of this research was investigation of PCOS on oocytes quality and p...
[ 6 ] - Histomorphometrical study of the effect of platelet-rich plasma therapy on the damage caused by the Torsion-Detorsion testicular in mice
Background & Objectives: Testicular Torsion is one of the emergency cases which is caused by twisting of the spermatic cord; Detorsion leads to the recirculation of blood and ultimately, more damage occurs in testis. Platelet-Rich plasma (PRP) contains several growth factors and cytokines that can restore tissue and rebuild cells, so in this study, PRP was used to evaluate its effects. Material...
[ 7 ] - Potentiation Effect Of 5FU by Fragaceatoxin C Pore-Forming Toxin in MCF-7 Cell Line
Introduction: Chemotherapy has been restricted due to the high-dose side effects. In the present study, acceleration of the chemotherapeutic drug (5FU) entrance into MCF-7 cells has been explored by using a recombinant form of Fragaceatoxin C (FraC) pore-forming toxin. Methods: In this experimental study, the gene for FraC toxin was order from a commercial source and was sub-cloned into pET28a...
[ 8 ] - Crocin exerts improving effects on indomethacin-induced small intestinal ulcer by antioxidant, anti-inflammatory and anti-apoptotic mechanisms
Crocin is a plant-derived carotenoid and bears potent antioxidant property. Ranitidine (a histamine H2 receptor blocker) is used for peptic ulcer treatment. The present study was planned to investigate the effects of crocin and ranitidine on indomethacin-induced ulcer in small intestine of rats. Animals were randomized into two major groups including indo-methacin (10.00 mg kg-1...
[ 9 ] - EVALUATION OF IN VITRO FERTILIZATION POTENTIAL AND SPERM PARAMETERS IN ACRYLAMIDE TREATED MICE
Background & Aims: Today, with lifestyle changes, the tendency to use packaged food, street food, and fast food has increased, and most of them contain Acrylamide. One of the most important side effects of Acrylamide is toxicity in the reproductive system. In this study, the long-term effects of Acrylamide on in Vitro Fertilization (IVF) and sperm parameters were investigated. Materials & ...
[ 10 ] - Cloning and Bioinformatics Analysis of the Gene Encoding Transcription Factor MYB44 of Sunflower (Helianthus annuus L.) under Salt Stress Conditions
Sunflower oilseeds (Helianthus annuus L.) are widely used around the world. Soil salinity negatively affects many morphological and physiological traits of sunflowers. Oil seed sunflower line tolerant to salinity stress (AS5305) was planted in normal and salinity stress conditions in a completely randomized design with two biological replications in a controlled environment. Salinity was applie...
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