Mohamad Hosein Nasr Esfahani

Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

[ 1 ] - MiR-9-5p and miR-106a-5p dysregulated in CD4+ T-cells of multiple sclerosis patients and targeted essential factors of T helper17/regulatory T-cells differentiation

Objective(s): Multiple sclerosis (MS) is considered as a chronic type of an inflammatory disease characterized by loss of myelin of CNS.Recent evidence indicates that Interleukin 17 (IL-17)-producing T helper cells (Th17 cells) population are increased and regulatory T cells (Treg cells) are decreased in MS. Despite extensive research in understanding the mechanism of Th17 and Treg differentiat...

[ 2 ] - Embryonic stem cells derived cardiomyocytes are a suitable model for assessment of cardiotoxic effects of doxorubicin and other drugs

Introduction: Doxorubicin is frequently used for treatment of several types of cancer. Doxorubicin cardiac toxicity has limited the use of this drug. Corticosteroids may prevent doxorubicin induced cardiotoxicity. Therefore the aim of this study was to evaluate mouse embryonic stem cells derived cardiomyocytes as a model to evaluate the effect of Doxorubicin and dexamethasone. Methods: Mouse ...

[ 3 ] - مقایسه تاثیر تمرین اینتروال شدید و تمرین استقامتی کم شدت بر بیان ژن Hig2 و محتوای چربی درون کبدی رت‌های مبتلا به کبد چرب غیر الکلی (NAFLD)

زمینه و هدف: بیماری کبد چرب غیر الکلی اصلی ترین بیماری کبدی است که بروز آن با عادات ناصحیح غذایی و اضافه وزن ارتباط دارد. هدف این پژوهش مقایسه اثرات 8 هفته تمرین اینتروال شدید (HIIT) و تمرین استقامتی کم شدت (LIET) بر بیان ژن Hig2 و محتوای تری گلیسیرید کبدی رت‌های مبتلا به کبد چرب (NAFLD) بود. روش بررسی: چهل رت نر ویستار به طور تصادفی در چهار گروه مساوی تقسیم شدند. گروه های کنترل، LIET و HIIT که...

[ 4 ] - Effects of assisted oocyte activation with calcium- ionophore and strontium chloride on in vitro ICSI outcomes

Objective(s): Failed fertilization after intra-cytoplasmic sperm injection (ICSI) is mainly attributed to failed oocyte activation and can be overcome by artificial oocyte activation (AOA). The present study aims to compare in vitro outcomes of ICSI following two different assisted oocyte activation chemical procedures (SrCl2 and Ionomycin) in sibling oocytes of ICSI candidates.Materials and Me...

[ 5 ] - شناخت بیولوژی مرگ سلولی اسپرم

زمینه و هدف: مرگ سلولی یکی از مباحثی است که در سال‌های اخیر توجه فراوانی را به خود جلب کرده است. در شرایط طبیعی سلول‌ها با استفاده از مسیرهای مختلف مانند آپوپتوز، اتوفاژی، پاسخ شبکه آندوپلاسمی و غیره این تعادل را حفظ می‌کنند. کوچک‌ ترین نقص در حفظ این تعادل می‎تواند منجر به بیماری‌های مختلف ازجمله ناباروری در مردان گردد. از آنجایی ‌که علت بسیاری از موارد ناباروری در مردان نامشخص است، دانشمندان ...

[ 6 ] - سانتروزوم و نقش آن در گامتوژنز و تکوین جنینی

زمینه و هدف: سانتروزوم ها تنها اندامک های بدون غشا در سلول های مهره داران هستند و در اکثر سلول ها از 2 ساختار اصلی، سانتریول ها و ماده ی پری سانتریولی تشکیل شده اند. در طی تکوین تغییرات عمده ای در ساختار و مورفولوژی سانتروزوم ها رخ می دهد که مهم ترین این تغییرات در طی فرایند گامتوژنز، لقاح و تکوین اولیه جنین است. هدف این مقاله مروری آشنا شدن با ساختار و ترکیبات سانتروزوم، نقش آن در گامتوژنز و ...

[ 7 ] - پیش بینی اثر گذاری miR-146, miR-142 و miR-150 در تمایز سلول های +naive CD4 به سلول های Th17 در بیماری مولتیپل اسکلروزیس

زمینه و هدف: miRNAها دسته ای از RNA های غیر کدشونده هستند که در فرایندهای مختلف سلولی ایفای نقش می کنند. اخیراً تغییر بیان این مولکول ها در بیماری های بسیاری گزارش شده است. متأسفانه بیماری مالتیپل اسکلروزیس امروزه به عنوان یک بیماری خود ایمن در ایران رو به افزایش است. سلول های Th17 مهم ترین سلول ها در این بیماری می باشند. در این مطالعه ما به دنبال نقش احتمالی miRNA های miR-142، miR-146a و miR-1...

[ 8 ] - Noscapine Inhibiting the Growth and Angiogenesis of Human Eutopic Endometrium of Endometriosis Patients through Expression of Apoptotic Genes and Nitric Oxide Reduction in Three-Dimensional Culture Model

Noscapine is a natural alkaloid with anti-angiogenesis activities. The aim of the present study was to examine the effect of noscapine on eutopic endometrium of endometriosis patients (EEE) and normal endometrium (NE)in a three-dimensional (3D) culture model. In this experimental in-vitro study, EEE (n = 8) and NE (n = 8) biopsies were taken from 16 reproductive aged women. The biopsie...

[ 9 ] - Noscapine Inhibiting the Growth and Angiogenesis of Human Eutopic Endometrium of Endometriosis Patients through Expression of Apoptotic Genes and Nitric Oxide Reduction in Three-Dimensional Culture Model

[ 10 ] - Effect of Vitamin D3 on Mitochondrial Biogenesis in Granulosa Cells Derived from Polycystic Ovary Syndrome

Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder diagnosed by anovulation hyperandrogenism.Hyperandrogenism increases apoptosis, which will eventually disturb follicular growth in PCOS patients.Since mitochondria regulate apoptosis, they might be affected by high incidence of follicular atresia. This may causeinfertility. Since vitamin D3 has been shown to...

[ 11 ] - An Overview of The Globozoospermia as A Multigen-ic Identified Syndrome

Acrosome plays an integral role during fertilization and its absence in individuals with globozoospermia leads to failure of in vitro fertilization (IVF) and oocyte activation post-intracytoplasmic sperm injection (ICSI). A variety of processes, organelles and structures are involved in acrosome biogenesis including, trans-Golgi network (TGN), acroplaxome and cellular trafficking. This review a...

[ 12 ] - Molecular Evidence of Chlamydia trachomatis Infection and its Relation to Miscarriage

Objective Chlamydia trachomatis (CT) infection is the most common sexually transmitted disease in the world that can persist and also ascend in the genital tract. This intracellular and silent infection is related to some adverse pregnancy outcomes, such as miscarriage. The aims of this study were to explore the best CT screening tests using blood and vaginal samples and to investigate the corr...

[ 13 ] - N-Acetylcysteine Compared to Metformin, Improves The Expression Profile of Growth Differentiation Factor-9 and Receptor Tyrosine Kinase c-Kit in The Oocytes of Patients with Polycystic Ovarian Syndrome

Objective Paracrine disruption of growth factors in women with polycystic ovarian syndrome results in production of low quality oocyte, especially following ovulation induction. The aim of this study was to investigate the effects of metformin (MET), N-acetylcysteine (NAC) and their combination on the hormonal levels and expression profile of GDF-9, BMP-15 and c-Kit, as hallmarks of oocyte qual...

[ 14 ] - Comparative stepwise pattern of reactive oxygen species production during in vitro development of fertilized and nuclear transferred goat embryos

Objective A unique feature of embryo metabolism is production of reactive oxygen species (ROS). It is well established that during in vitro culture, ROS levels increase over normal ranges observed for embryos developed in vivo. This study evaluates and compares the stepwise pattern of ROS production during in vitro development of reconstructed goat embryos produced by zona-free method of somati...

[ 15 ] - Investigation of Personality Traits between Infertile Women Submitted to Assisted Reproductive Technology or Surrogacy

Objective Personality traits affect human relationships, social interactions, treatment procedures, and essentially all human activities. The purpose of this study is to investigate the personality traits -including sensation seeking, flexibility, and happiness - among a variety of infertile women who were apt to choose assisted reproductive technology (ART) or surrogacy. MaterialsAndMethods Th...

[ 16 ] - Expression profile of developmentally important genes in pre- and peri-implantation goat embryos produced in vitro

Background Little is understood about the regulation of gene expression during early goat embryo development. This study investigated the expression profile of 19 genes, known to be critical for early embryo development in mouse and human, at five different stages of goat in vitro embryo development (oocyte, 8-16 cell, morula, day-7 blastocyst, and day 14 blastocyst). MaterialsAndMethods Stage-...