Validation of Integral System Yeast Plus for rapid identification and determination of antifungal susceptibility profile of clinically important Candida species

Precise identification of microorganisms involved in candidiasis together with antifungal susceptibility evaluation could help clinicians to prescribe appropriate medicine, especially in patients with critical conditions. The present study has been conducted to evaluate discriminatory power of Integral System Yeast Plus (ISYP) for rapid identification and determination of antifungal susceptibility of clinically important Candida species (sp.). Validation of ISYP results was performed using molecular and Broth Micro Dilution (BMD) assays. Referring to the present results, it can be said that ISYP was to some extent successful for identification of C. albicans isolates. Major misidentification was observed in cases of non C. albicans sp. (NAC) in comparison with sequencing results. The relatively unsatisfied outcome of ISYP performance was correlated to antifungals susceptibility assay as well.  It is noteworthy to emphasize the potential advantages of ISYP for simultaneous identification of Candida sp. together with antifungal susceptibility evaluation that brings hope to the defeat of severe complications. But an essential defect was observed in both identification and antifungal susceptibility tests.