The proliferation of fibroblast cells on the polycaprolactane-chitosan-tannic acid scaffold

Background and Objective: Tissue engineering is a new method for replacing damaged tissue components in order to improve its function. In this method, a porous scaffold mixed with polysaccharide and synthetic antioxidants is first produced and then stem cells are cultured inside it. In this study, the polycaprolactane-chitosan-tannic acid scaffold was used to reproduce the amount Fibroblast cells are evaluated. Materials and Methods: In this experimental study, polycaprolactane, chitosan powder and tannic acid scaffold were prepared for growth of fibroblast cells. Subsequently, the following groups were designed: Group 1: Polycaprolactane scaffold Group 2: Polycaprolactane-chitosan scaffold Group 3: Polycaprolactane-tannic acid scaffold Group 4: Polycaprolactane-chitosan-tannic acid scaffold. The human foreskin was prepared and the dermal layer fibroblast cells were isolated after laboratory tests, then the cells were placed in cell culture flasks with DMEM medium and stored in a 5% CO 2 incubator. Ten thousand cells of fibroblast cells seed in 96 wells containing DMEM solution and scaffolds of the subgroups, followed by the proliferation and survival of fibroblast cells using the MTT method, and also using the SEM microscope, the penetration of fibroblast cells on the scaffold and also in order to review of the chemical groups in the polymers was performed using a FTIR spectrometer. The results were analyzed by SPSS software; ANOVA and Tukey's post hoc test after the data were analyzed uniformly. Results: The mean survival rate of fibroblast cells based on MTT assay in 24 hours in the polycaprolactane-tannic acid scaffold group was significantly higher than that of the polycaprolactane scaffold group (P <0.05). The results also show that The mean survival rate of cells based on MTT test in 24 hours in the polycaprolactane-chitosan-tannic acid scaffold group was significantly higher than that of the polycaprolactane scaffold group (P <0.05). Conclusion: The results of this study showed that the polycaprolactane-chitosan-tannic acid scaffold, probably hydrophilicity and biocompatibility of chitosan and tannic acid, could be a suitable scaffold for the activity of fibroblastic cells in the scaffold.