The Effects of Glycogen Syntase Kinase-3 β Inhibitor on The Ostogenic Differentiation of Unrestricted Somatic Stem Cells (Ussc)

Purpose: Bone defects caused by injuries, trauma, cancer, surgical operation or joint dislocation have great impacts on general health. Considering that Wnt signalling is one of the important regulators of the stem cell control, we sought to examine if Wnt activation affects the osteogenic differentiation of the USSCs.Materials and Methods: In this study, we have treated USSCs cultured in osteo genic media with a specific GSK3b inhibitor, BIO, at days 4, 7, 14 and 21 in culture. They were then assayed for MTT, bone formation matrix and RT-PCR.Result: Our results showed that up to it day 14, BIO increases the gene expression of alkaline phosphatase and Runx2; however decreases their expression as of day 14 on. The result of bone matrix assay showed that BIO decreases the synthesis of bone matrix as well that of the viability of the differentiating USSCs.Conclusion: In general these results indicate that BIO as a GSK-3b inhibitor, and therefore Wnt pathway activator, decreases the osteogenic differentiation of USSCs.