The Effect of Nifedipine and Verapamil on Kidney Histology Rat

Purpose: The aim of this study was to evaluate the effect of verapamil and nifidipine on diabetic kidney in rats. Materials and Methods: In this experimental study, 6 groups of rats were studied, group I; intact (sham) group II; diabetic (control), group III and IV; diabetic treated with verapamil or nifedepine group V and VI normal rat which recieved verapamil or nifedipine. Diabetic rats (control group) were received 50 mg/kg streptozotosin (STZ). Verapamil was used 40mg/kg and nifedipine 10mg/kg/dI. The animals were received nifedipine and verapamil from 2 months after diabetes induction. After 2 months the rats were anesthetized and their left kidney was removed. The kidneys were weighted and then dissected for hydraxy prolyne evalution and histological study. The tissues were fixed by 10% formaldehyde and after blocking 5-micron sections, stained by PAS×H×E and three chrome masons methods. The diameters of Glomerulus's, proximaltubule, distal tubule and coilecting ducts were measured in all groups. Results: The mean of kidney weights have not shown significant change in all groups. The hydroxyproline was reduced (p<0.001) in diabetic rats treated with verapamil (40 mg/kg/dI) and nifedipine (10mg/kg/dI). There was no statistical significant change in diameter of glumerole, proximal tubule, distal tubul & collection tubel between the two normal & diabetic rat groups. The diameter of glumerole, proximal tubule, distal tubule and collection tubule have no significant change between normal and diabetic rats. Group although the diameter of distal and collecting tubules in normal rats were in created (P<0.01). After receiving the verapamile and nifedipine. In diabetic animals Just the diameter of distal tubules was increased (p<0.05). The pathological fixings were shown to be increased in basal membrane and member of collagen fiber in diabetic group. Conclusion: Verpamil and nifedipin could decrease interstitial fibrous tissue in all diabetic groups during treatment period. But longer period is needed for atrophy and another chaner in kidney.