The Effect of Isosorbide and Garlic Supplementation on Protein Levels and Gene Expression of PLA2 at the Heart Tissue in Female Rats with Hypertension After a Period of Endurance Training

Aims: Hypertension is a polygenic disease that is associated with the interaction of genetic and environmental factors.The aim of this study was to investigate the effect of Isosorbide and Garlic supplementation on protein levels and gene expression of PLA2 at the heart tissue in female rats with hypertension after a period of aerobic training. Materials & Methods: In this experimental study,  40female Wistar rats weighing 180-220 g were randomly divided into 8 groups: healthy control, sham, blood pressure induction (Hyper), garlic, endurance training, endurance training -garlic, isosorbide and endurance training -isosorbide. The rats suffered from hypertension after dietary period and 10 mg/kg body weight L_NAME injection, 6 days a week for 8 weeks. Experimental groups received 40 mg/kg body weight of isosorbide and 50 mg/kg body weight garlic supplement for six weeks. The endurance training program was performed at speed of 20-30 m/min for 20 to 35 minutes, 5 sessions per week for 6 weeks. Protein levels and expression of PLA2 heart tissue were measured using ELISA kit and Real Time PCR. Results: The results showed that the level of PLA2 Protein at the heart tissue in Isosorbide (P=0.043) and Isosorbide - training (P=0.035) groups was significantly lower than the blood pressure induction group. Also, the gene expression level of PLA2 at the heart tissue in the garlic, training, garlic - training, Isosorbide and Isosorbide - training significantly decreased that of the hypertension group (P=0.0001). Conclusion: According to the findings of this research,it seems aerobic training, garlic and isosorbide and their combined intervention can help to improve the factors involved in hypertension.