the effect of isoproterenol on protein electrophoretic pattern of rabbit salivary Glands

Isoproterenol is a potent B-adrenergic agonist which its effect on rabbit salivary Glands has been studied in this research.two groups of male albino rabbits(five in each group with the mean weight of 1000+_300 gr) were injected with 20 mg/kg of  isoproterenol intrapritoneally each day for fifteen days and one similar group(n=5) was chosen as control group.in one of the test groups in addition to normal diet (day food) animals were fed by liquid diet.increase in the weight of parotid salivary Glands was five folds in test groups as compared to control group(p<0.01).also,twelve folds increase in total protein content of the gland was observed in test groups as compared to control group(p<0.01).the increase in submandibular gland weight was significant but not as much as that in parotid gland(p<0.01).the total protein of submandibular glands of those experimental animals which were fed with solid diet was 2.5 folds as compared to control group(p<0.01).the total protein of the other test group fed with liquid diet showed a non-significant increase in comparison to control group.the increase in submandibular gland weight was significant in this group compared to control group(p<0.01).regarding sublingual gland, there was just significant increase in weight of gland in the solid diet group(p<0.01).however the increase in total protein content of sublingual glands of experimental groups was not significant compared to control group.sodium didecyl sulfate polyacrylamide gel electrophoresis of parotid gland revealed protein bands with molecular weight of about 14,44,58,96,and 98 KD in experimental groups compared to control group.electrophoretic pattern of submandibular gland of test groups showed protein bands with 20.KD which probably is a kind of proteins.since isoproterenol was not effective on sublingual gland,significant differnce in its electrophoretic pattern was not observed.