The Effect of Eight Weeks of Resistance Training with Tribulus Terrestris Extract on the Apoptosis Mechanism in the Heart Tissue of Rats Exposed To Stanozolol

Background: Artificial intelligence analysis based on the gene list obtained from the DisGeNET database identified the important genes involved in the heart damage process. Data enrichment highlighted the apoptosis signaling pathway as a vital pathway in cardiovascular risk. Hence, we estimated the binding affinity of chemical and plant bioactive molecules for cytochrome-c protein. Here, we performed to evaluate the effect of eight weeks of resistance training (RT) with Tribulus Terrestris (TT) consumption on the mechanism of apoptosis in the heart tissue of rats exposed to stanozolol. Methods: Thirty-five male rats were divided into seven groups: (1) Control, (2) Stanozolol (ST), (3) ST + 100 mg / kg TT, (4) ST + 50 mg / kg TT, (5) ST + RT (SRT), (6) S + RT + 100 mg / kg T (SRTT100), and (7) ST + RT + 50 mg / kg T (SRTT50). Differential gene expression was measured by q-RT-PCR. In bioinformatics analysis, the apoptosis signaling pathway was defined as a critical process in heart damage. In addition, adverse effects of Tribulus Terrestris and stanozolol on heart tissue were detected through the apoptotic pathway by molecular docking. Result: Resistance training along with 100 mg/kg reduced CRP and cytochrome-c Moreover, 100 mg/kg TT as a more favorable effect than 50 mg/kg TT Conclusion: we showed the beneficial effects of Tribulus Terrestris, the plant’s bioactive compound that can reduce cardiovascular risks by impairing the formation of apoptosome assemblages and inflammation.