The Effect of Eight Weeks of Endurance Training, Growth Hormone Injection and its Lipolytic Fragment (AOD9604) on CK18 and Liver Enzymes of NAFLD-induced Mice Induced by High-fat Diet

Aims: Fatty liver disease and overcoming it is one of the concerns of today's society, so the aim of this study was to investigate the effect of eight weeks of endurance training, growth hormone injection and its lipolytic fragment (AOD9604) on CK18 levels and liver enzymes of mice induced in fatty liver. Materials & Methods: In This experimental study, 28 male mice were, randomly, divided into four groups (n=7): control (C), Exercise (E), Exercise + Fragment (EA), Exercise + Growth Hormone (EGH) group. The Medium intensity endurance training program was performed for eight weeks and 5 sessions with an intensity of 50% VO2max of per week. The somatropin and fragment injection protocols were 1 mg and 250 picograms per kilogram of body weight, respectively. Samples were evaluated 48 hours after the last training session. Statistical analysis of the data using the software SPSS version 26, using OneWay ANOVA and post hoc Tukey tests at the significant level (P<0/05) was carried out. Findings: CK18 showed a significant decrease compared to the control group only in group E (p=0/00). CK18 values were significantly higher in the EGH group compared to the E (p=0/00) and EA (p=0/04) groups. HOMA-IR index had a significant decrease in E (p=0/00) and EA (p=0/03) groups, but no significant changes in EGH group. Changes in AST were not significant in any of the groups. ALT levels in all three groups of E (p=0/00), EGH (p=0/03) and EA(p=0/00) were significantly lower than the control group, but the changes between groups were not significant. Conclusion: endurance training has produced a more effective response in improving NAFLD markers than GH and fragment peptides, Growth hormone injection can have negative consequences on some indicators of this abnormality.