THE EFFECT OF CONTINUOUS TRAINING ON RATE OF CASPASE3, BAX AND BCL-2 PROTEINS IN VASCULAR OF INDUCED POISONING INDUCED BY DIFFERENT DOSES OF HYDROGEN PEROXIDE

Background & Aims: Apoptosis is organized cellular death, induced by various physiological and pathological factors. Therefore, the purpose of this study is to investigate the effect of continuous training on rate of Caspase3, BAX and BCL-2 proteins in rats vascular by induced poisoning due to various doses of hydrogen peroxide. Materials & Methods: 40 mature male wistar rats were randomly assigned into 5 groups including 8 rats in each group. The groups were injected by: 1-Intraperitoneal injection of 1mM H2O2, group (H1), 2- Intraperitoneal injection of 2mM H2O2, group(H2), 3-Intraperitoneal injection of 1mM H2O2 with regular exercise, group (H1E), 4- Intraperitoneal injection of 2mM H2O2 with regular exercise, group (H2E), and 5-control group(C).The training groups performed regular training on the treadmill daily for 8 weeks, all of rats after the anaesthesia knocked out and the BAX, BCL-2 and Caspas3 proteins ratio were determined and measured by ELISA technic. The ANOVA Two Way test was used to determine the effect of each of the independent variables on the indicators. Also, the ANOVA One Way test of the Bonfroni follow-up test was used to examine the intergroup of each of the variables. And significance was considered as P≤ 00.05. Results: Continuous training in the rats showed that the amount of BAX protein did not decrease significantly compared to the treatment group with 1 and 2 mM of hydrogen peroxide. Exercise alone or with injections of 1 and 2 mM of hydrogen peroxide, increased the amount of protein BCL-2, which was not significant and decreased Caspase3, BAX, and BAX / BCL-2 ratios in both groups receiving one and two milliliters of hydrogen peroxide, but only changes in Caspase-3 (P = 0.014) and ratio BAX / BCL-2 (p = 0.0002) was significant in groups receiving 2 mM of hydrogen peroxide (P ≤ 0.05). Conclusion:Continuous exercises are likely to reduce the apoptosis of endothelial cells by reducing Caspase3, BAX / BCL-2 ratio and increasing BCL-2 which is likely to prevent vascular tissu damage.