The effect of caffeine-beet supplementation on NTproBNP and IL-6 following a course of aerobic activity in professional endurance runners

Background and Aim: Examining the physiological characteristics and recognizing the pathological factors of the heart during sports and endurance activities can provide a good background in identifying athletes who are at risk. NTproBNP has been introduced as a biological marker for the diagnosis of heart failure and disease progression in response to increased stress on the heart wall, increased volume, ventricular and atrial pressure, and other conditions such as ischemia or myocardial infarction and in response to various signals including stress. Increased myocardial infarction On the other hand, several studies have shown that elevated cytokines, particularly IL-6, severely modulate cardiac function and can lead to myocyte damage. Increased plasma IL-6 levels during exercise are associated with left ventricular dysfunction and are an important factor in the development of heart failure. The aim of this study was to evaluate the effect of caffeine-nitrate supplementation on NTproBNP and IL-6 following a period of aerobic activity in runners. It was an occupational profession. Methods: The method of this research is laboratory-experimental cross-sectional and cross-sectional, cross-sectional and three-blind, with targeted random selection of professional runners and with the aim of investigating the effect of caffeine and beet juice mixture containing nitrate on NTproBNP and IL-6 A course of aerobic activity was performed in professional endurance runners. 15 elite male runners with a mean age of 27.5 3. 3.8, mean height of 176.7 7 7, mean weight of 69.9 7. 7.8 and body mass index of 22.3 1.5 1.5 and maximum oxygen consumption (ml / kg / min) 4.6 سه 52.2 were selected and selected as 52.2 , Were divided into three groups: 1) high dose (9.6 mmol nitrate and 400 mg caffeine), 2) low dose (4.8 and 200) and 3) placebo. Subjects consumed the supplement twice daily with 250 cc of water for seven days before the test. Aerobic exercise included running on a treadmill for 5 km. Plasma concentration of NTproBNP was measured in picograms per ml (pg / ml) and by electrochemiluminescence immunoassay (Elecsys NT-proBNP 2010) with a sensitivity of 125 pg / ml. Plasma IL-6 was measured by BMS213HS kit with a sensitivity of 0.02 pg / ml, the results were shown in picograms per ml. Beet extract in powder form Beet powder Sun Say extract is completely herbal and organic without sugar, gluten and other preservatives and synthetic chemicals and made under license 14.10645 and caffeine in powder form made by Karen company and placebo was natural beet juice. It was evaluated by food quality control laboratory and determined as a placebo with a little basic nitrate. The supplements were combined in triplicate by the researcher's colleague in water and provided to the researcher as a solution for consumption. Random help supplement: 1) 9.6 mmol beet extract and 400 mg caffeine for high dose group, 2) 4.8 mmol beet extract and 200 mg caffeine for low dose group and 3) natural beet juice placebo for group Placebo, done Results: The results showed that supplementation had no significant effect on NTproBNP (p = 0.821), but decreased IL-6 (p = 0.001). Also, one session of exercise significantly increased the levels of NTproBNP (p <0.05) and IL-6 (p <0.05) and supplementation after seven days decreased the levels of NTproBNP (p = 0.071) and IL-6 (p = 0.092) followed by sports activities. Conclusion: Considering the increase in plasma levels of NTproBNP and IL-6 immediately after exercise intervention and their significant decrease seven days after intervention, it can be said that due to doubts about the increase in these parameters after exercise, An activity with such a temporal and intense nature does not seem to lead to harm. However, the greater intensity in competitions and the effect of repetition of this training pattern in the long run can not be ignored and it is suggested that in addition to interpreting the study results of plasma levels of NTproBNP and IL-6 in athletes, comprehensive cardiological studies and physiological factors affect Examine the secretion of these markers in other endurance sports activities to obtain more accurate information about the physiological function of athletes' hearts during endurance sports activities. Also, due to the lack of significant effect of caffeine nitrate supplement on NTproBNP and the significant reduction of this supplement to IL-6 after intense and short anaerobic exercise, it is not possible to be confident about taking caffeine-nitrate supplement to control inflammatory factors after intense anaerobic exercise. Commented; However, taking this supplement, due to its anti-oxidant properties and findings based on previous studies, will probably be somewhat beneficial for athletes who train hard. It may need to be used for a longer period of time to be more effective, and more studies are needed to find out more about the anti-inflammatory effects of caffeine-nitrate supplementation in humans. The limitations of this study were low number of samples, duration of sports intervention, lack of control over mental states and motivation of the subjects. Also, although the subjects were given a 24-hour reminder form, it was not possible to precisely control their feeding. In general, according to the findings of the present study and regardless of its limitations and considering the precautionary aspects, it can be recommended to young athletes who can use caffeine-nitrate supplement to modulate cardiovascular risk factors.