The Effect of Aerobic Exercises and Octopamine Consumption on Changes in Gene Expression of Caspase-9, NLRP3, AIM2 in Brown Adipose Tissue of Rats after Intoxication with Deep Fried Oil: An Experimental Study

Background and Objectives: Regular aerobic exercises along with herbal supplements can control inflammation. The aim of this study was to investigate the effect of aerobic exercises and octopamine consumption on gene changes of NLR family pyrin domain containing 3 (NLRP3), absent in melanoma 2 (AIM2) and caspase 9 in brown adipose tissue in rats after consuming deep fried oil. Materials and Methods: The present study was an experimental study. Forty male Wistar rats were randomly divided into groups of 8 included: healthy control, control poisoned with fried oil, poisoned + aerobic exercise, poisoned + octopamine, and poisoned + aerobic exercise + octopamine. The fried oil was fed orally to rats for 4 weeks. The dose of Octopamine was 81μmol/kg. The aerobic exercise groups also trained on the treadmill with increasing intensity for four weeks. Real time PCR and IHC methods were used to determine changes in NLRP3, AIM2, caspase 9 genes as well as changes in NLRP3 protein expression, respectively. Two-way analysis of variance was used to analyze the data. Results: Aerobic exercise caused a significant decrease in NLRP3 (p=0.010) and AIM2 (p=0.001) gene expression compared to the poisoned control group. Octopamine supplementation also significantly reduced NLRP3 (p=0.009) and AIM2 (p=0.001) gene expression. The interaction of exercise and aerobic exercise caused a significant decrease in NLRP3 protein (p=0.001). Conclusion: Applying 4 weeks of exercise or taking octopamine alone by controlling the inflammasome complex reduces cell death in brown adipose tissue, which is effective in browning and making adipose tissue more efficient. Key words: Apoptosis, NLRP3, AIM2, Brown adipose tissue, Aerobic exercise, Octopamine, Deep fried oil   Funding: This study did not have any funds. Conflict of interest: None declared. Ethical approval: The Ethics Committee of the Faculty of Veterinary Medicine, Sannandaj University approved the study (IR.MUK.REC.1398.242).   How to cite this article: Darvishzadeh N, Azizbeigi K, Etemad Z. The Effect of Aerobic Exercises and Octopamine Consumption on Changes in Gene Expression of Caspase-9, Nlrp3, Aim2 in Brown Adipose Tissue of Rats after Intoxication with Deep Fried Oil: An Experimental Study. J Rafsanjan Univ Med Sci 2021; 20 (1): 37-52. [Farsi]