Study on sexual reproduction and some morphological and pathological traits of Ophiognomonia leptostyla in Iran

Walnut anthracnose caused by Ophiognomonia leptostyla is the most important and prevalent fungal disease in most walnut growing areas in Iran. Seventy-five isolates of Ophiognomonia leptostyla, causing walnut anthracnose, were obtained from Juglans regia from various regions of Iran. In order to study the sexual reproduction of the fungus, the isolates collected from various parts of Iran were examined and perithecia were obtained from 11 regions from leaves and in eight isolates from oatmeal agar (OA). These isolates along with five sexually non-fertile isolates were purified as single-ascospore or single-macroconidium cultures. Protoperithecia were readily obtained from leaves collected more than one year back. Perithecia had one beak on leaves and up to four beaks on culture media. As the result 9.3% of isolates were found to be homothallic. برگی با قدمت بیش از یک سال به دست آمدند. تعداد گردن آسکوکارپ در برگ یک و در محیط کشت تا چهار عدد متغیر بود. در این مطالعه 3/9%  جدایه‌ها هموتال بودند.