Study of Antifungal effect of Punica Granatum alcoholic extract on Candida Albicans (invitro study)

Abstract Background & Aim: Oral diseases affect the majority of the population and can affect a person’s overall health. It is reported that punicagranutum contain phytomedicinecapabale  of  suppressing oral pathogens associated with fungal diseases. The aim of this study is the assessement of punicagranutm extract antifungal activity on Candida albicans. Material and methods: This study was performed in Qazvin University of Medical Sciences in 2019 The70% ethanol extract of punicagranutum was purchased from the Research Institute of Chemical Industry Development and then made in 1- 1/2048 mg/ml concentrations.The antifungal activity of the extractwas examined by determining the MIC (minimum inhibitory concentration) using the Macrobroth dilution method, means macroscopic examination of the test tubes containing serial dilutions of extract and candida albicans to determine the growth or lack of growth of microorganisms through the ¬eye, and MFC (Minimum Fungicidal Concentration) was determined by preparing the subculture of the contents of all of the undeveloped tubes in a MIC experiment in a solid culture. Also, the non-growth aureole diameter with Agar well diffusion method was obtained by preparing wells in a solid culture medium  And pouring different dilutions of the extract into it and measuring the non-growth aurachr('39')s diameter.Data were analyzed using ANOVA, post-hoc tests  (p<0.05). Results: Results showed that the ethanol extract of punicagranutumhad inhibitory effect in concentration of   mg/ml and fungicidal effect in concentration of  mg/ml in which the most of this effect is due to punica granutumbut not theethanol.Also, the mean of maximum non-growth aureole diameter was 21 mm at a concentration of 1 mg / ml. Conclusions: Comparison of the results of ethanol control group and test group showed that inhibitory and fungicidal effect of hydroalcoholic extract of punicagranutum is not due to Ethanol and the punicagranutum and Ethanol extracts of punicagranutum has antifungal effect on Candida albicans.