Protective and modulatory effects of royal jelly used against the induced changes in silver nanoparticles on anxiety behavior and hippocampal tissue of the hippocampus of male rats

Introduction: Since silver nanoparticles have harmful effects on the body and in addition to extensive applications in technology, The present study aimed to evaluate the Anxiety and tissue of hippocampus of rats treated with silver nanoparticles were performed Materials and Methods: In this experimental study, 40 adult male Wistar rats weighing 250-200 gr (5-6 months) in 4 groups of 10 rats including: Healthy control, Royal jelly receiving, silver nanoparticle and royal jelly, silver nanoparticle receiving was performed. The rats were gavage silver nanoparticles and royal jelly at doses of 30 and 100 mg/kg for 28 days, respectively. After a 24-hour break, anxiety behavior was assessed using plus-maze behavioral test. Hippocampus tissue was collected for histological studies. Results: The results of anxiety test showed Royal jelly significantly increased the number of entry and presence percentage of open arm compared to other groups (p<0.01) Also, administration of silver nanoparticles significantly decreased the number of entry and presence in the open arm compared to other groups (p<0.05). Results of hippocampus tissue changes showed that Royal gel reduces the density of picnotic neurons in the royal jelly group and silver nanoparticles compared to the silver nanoparticle group. The difference between the control and experimental groups was not significant (p>0.05). Conclusion: according to the findings of this study, it can be concluded, Royal jelly can reduce anxiety in rat due to its antioxidant properties