Polymorphism in Prolactin and PEPCK-C Genes and Its Association with Economic Traits in Native Fowl of Yazd Province
نویسندگان: ثبت نشده
چکیده مقاله:
The objective of the present study was to investigate the polymorphism of prolactin promoter and cytosolicphosphoenol pyruvate carboxykinase (PEPCK-C) intron 3 to exon 3 regions, and its association with economictraits in native fowl of Yazd province. These traits consisted of body weight at 8 (BW8) and 12 (BW12) weeks of age, age at sexual maturity (ASM), weight at sexual maturity (WSM), mean egg weight at28, 30, and 32 weeks (MEW), and the number of eggs during the first 12 weeks of laying period (EN). Bloodsamples were collected from 159 pedigreed fowl at native fowl breeding center of Yazd province, and DNAwas extracted from the samples according to saltingout protocol. PCR amplification together with restrictionfragment length polymorphism was used to identify different genotypes of prolactin and PEPCK-Cgenes. The effect of prolactin genotypes on economic traits was analyzed using general linear model. A 24-bp indel (insertion or deletion) at nucleotide position (np) 358 was identified, but no polymorphism wasfound for PEPCK-C. Based on our results, the frequency of I and D alleles were 0.761 and 0.239,respectively. Frequencies of II, ID and DD genotypes were 0.566, 0.389 and 0.044, respectively. GenotypesII and ID were significantly associated with incresased EN (P<0.01). Meanwhile, the genotypes of the 24-bpindel site were not significantly associated with BW8, BW12, ASM, WSM and MEW (P> 0.05). The results ofcurrent study showed that using information of genes related to egg production could be used to improve theperformance of native fowl of Yazd province.
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عنوان ژورنال
دوره 8 شماره 3
صفحات 172- 177
تاریخ انتشار 2010-07-01
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