P-82: Study The Anti-Inflammatory Effect of 1-(1-(4-Chlorobenzoyl)-6-Methoxy-2-Methyl-Acetohydrazide on In Vitro Sperm Activation of Astheno-leukozoospermic Infertile Men

such as varicocele, inflammation, and smoking, are well known to influence the balance between radical production and antioxidant defense. The objective of the present study was to select an efficient Non-steriod Anti- inflammatory Drugs (NSAIDs) concentrations of sperm activation in vitro for patient complaining from astheno-leucozoospermia. In vitro sperm activation was performed on seminal fluid samples by using simple layer technique for three incubation period (30, 45, 60) in Earl's medium to select the efficient concentration and incubation period. NSAIDs inhibit the cyclooxgenase (COX) activity resulting in decreased synthesis of prostagland in, leukotriene and thromoxane precursors. Materials and Methods: Semen samples were obtained from Astheno-leucozoospermia infertile men. This study includes 40 subfertile male partners between July 2013 to April 2014, from couples who had consulted the infertility clinic of the Babil hospital of maternity (Hilla city/IRAQ).The Semen specimens were divided into four fractions. First part was control, 0.5ml of liquefied semen mixed with 0.5 ml Earl's medium and incubated at 37°C for three incubation periods. Second part (antibiotic con.1), 0.5ml liquefied semen was mixed with 0.5ml Earl's medium supplemented with 0.02 mg/ ml antibiotic and incubated at 37°C for 30,45,60 incubation periods. Third part (antibiotic con. 2), 0.5ml liquefied semen was mixed with 0.5ml Earl's medium supplemented with 0.04 mg/ml antibiotic and incubated at 37°C for 30 minutes. Fourth part (antibiotic con. 3), 0.5ml liquefied semen was mixed with 0.5 ml Earl's medium supplemented with 0.06 mg/ml antibiotic and incubate at 37°C for three incubation period. After semen specimen treated in all fractions, they were examined and assessed for macroscopic and microscopic changes. Results: The treatment of low-level leukocytospermia with present antibiotic shows no significantly enhancement in the semen parameters among the treated patients. Low-level leukocytospermia resolved in good percentage of the treated groups. Conclusion: The results of this study indicated that adding present antibiotic to the culture medium for in vitro sperm activation leads to non significant improvement in certain sperm function parameters.