P-78: Role of Allopurinol, as An Antioxidant Factor, in Increasing The Number of Received Oocytes and Embryos, and Reduce Apoptosis after Heterotopic Transplantation Mouse Ovarian Tissue

Background: Ischemia and reperfusion after transplantation is the main problem which decreases follicular density in the grafted ovarian. Many sources of free radicals such as xanthine oxidase were generated during ischemia. In this study, we used allopurinol as xanthine oxidase inhibitor to reduce ischemia-reperfusion injury, to increase received oocytes and embryos, and to decrease Apoptosis after ovarian heterotopic autotransplantation in mice. Materials and Methods: Six-week-old (NMRI) mice were used in this study. Ovarian tissues were grafted into back muscle and then collected three weeks later. A total of two groups were included in this experiment: sham group (without treatment factor) and allopurinol treatment group (5 mg/kg/day). We investigated number of received oocytes and embryos as well as rate of Apoptosis in grafted ovary (immunohistochemistry assay). Data are present as the mean ± SEM and were analyzed by one way ANOVA and Kruskal-Wallis test. A probability of p<0.05 was considered to be statistically significant. The protocols approved by Ethics Committee of Royan Institute (Tehran, Iran). Results: The number of received oocytes and embryos significantly increased in Allopurinol group compared with sham group (p<0.05). Rate of Apoptosis decreased in allopurinol compared with sham group. Conclusion: Xo plays an important role in generating superoxide anions. So allopurinol, as a XO inhibitor, reduces superoxide anions production such as O2- and H2O2. We demonstrate that allopurinol plays an important role in order to decrease ischemia injury and to increase survival rate for follicles. Therefore, number of received oocytes and embryos are increased and apoptosis injury is decreased. These observations confirm that follicles start developing rather than remaining static after transplantation.