P-64: Anthocyanin Effects on Sheep Oocytes In Vitro Maturation in The Presence of cAMP Modulators.

Background We have previously reported the effect of Anthocyanin and cAMP modulators on maturation and apoptosis of sheep oocytes. In this study, we investigated the reduction capacity and apoptotic gene expression results at MII sheep oocytes. MaterialsAndMethods Good ovine Cumulus-oocyte complexes (COCs) were transferred to Pre-IVM medium, then to two type IVM mediums: 1) 0.1μg/ml Delphinidin Chloride supplemented SPOM medium and 2) Simple SPOM medium for 24 hours. After maturation period, MII oocytes with first polar body were selected. Immunofluorescence microscopy staining was used to evaluate intracellular GSH and ROS content of MII oocytes. In addition, the expression rate of some apoptotic genes (Bcl-2, Bax, Caspase 3, P53, C-myc) was investigated by Real-time PCR in MII oocytes. Statistical analyses were carried out by T test. Results Anthocyanin treatment during IVM increased intracellular glutathione (GSH) levels and significantly reduced reactive oxygen species (ROS) (19.97 ± 1.29 and 26.77 ± 2.6 respectively, P