P-62: Evaluation of Genetic Alterations in Exons 3 and 28 of SPEF2 Gene in Infertile Men with Short Tail Sperm Compared toControl Subjects

Background: This study aimed to investigate the inhibitory effects of simultaneous formaldehyde (FA) and noise exposure on the sperm kinematic parameters sexual hormones in male mice. Materials and Methods: Forty eight NMRI adult male mice were randomly assigned to control group and experimental groups which were exposed to formaldehyde vapour (10 ppm) (F), noise (100 dB) (N), and simultaneous formaldehyde with noise (NF), respectively for 10 days (8 hours a day). Mice were sacrificed 24 hours after exposure (short-term effects) and the rest of mice were sacrificed 35 days after the end of exposure (long-term effects). Sperm analyzed by computer aided sperm analysis system. Plasma concentrations of testosterone, LH, FSH were measured. Results: The results of short-term analysis showed that the serum testosterone in all exposure groups were decreased significantly compared to the control (p<0.001). In long-term analysis, the percentages of progressive motile sperm groups N, F, and NF were 33.73 ± 3.4, 26.65 ± 1.61, and 14.78 ± 4.65 respectively which had a significant decrease (p<0.05) compared to control group (44.47 ± 2.88). The progressive motile sperm in simultaneous exposure group was less than N and F groups (p<0.001). Conclusion: Thus, it can be anticipated that simultaneous exposure to formaldehyde and noise in workplaces can increase the possibility of damage to reproductive functions.