P-50: Evaluation of Sperm Parameters and Chromatin Abnormalities in Infertility with CASA and Chromatin Dispersion Test

Background: Although sperm selection according to morphologic and motility characteristic, made to some what the fertility successful, but sperm’s normal morphology cannot demonstrate sperm DNA competence. Since the health of sperm DNA is affecting the results of assisted reproductive technology; the aim of this study is to evaluate semen parameters measured by CASA with sperm chromatin abnormalities in Taleghani Medical Center in 1390 and 1391. Materials and Methods: Case-control study was conducted. Subjects who had a diagnosis of infertility and a control group who had previous fertility. Semen samples were taken and sperm parameters such as motility and morphology evaluated with CASA and DNA fragmentation was assessed by chromatin dispersion test. Protamine deficiency was evaluated by chromomycin A3 staining. Parameters in both groups were analyzed statistically. Results: The results showed a significant correlation between protamine deficiency, DNA fragmentation and sperm parameters. Head and neck anomalies showed the maximum significant correlation with percentage of sperm DNA fragmentation; but tail anomalies even occur with percentage of DNA competence. Conclusion: Protamine deficiency and damage in sperm DNA in infertile cases was more than fertile cases. In cases with high DNA damage ratio, sperm parameters were poorer and the fertility was less, so CASA method cans analysis sperm parameters with high precision.