P-219: GDF9, BMP15 and Their Receptors Expression During In Vitro Culture of Mouse Vitrified Ovarian Tissue Derived Preantral Follicles
نویسندگان
چکیده مقاله:
Background: In vitro culture (IVC) of isolated preantral follicles from cryopreserved ovarian tissue might be an efficient method for enhancing mature oocytes in patients who are exposed to infertility. Materials and Methods: Ovaries of 13-day old NMRI mice were removed and randomly placed into control,needle immersed (NIV) and solid surface vitrification (SSV) groups. For vitrification, ovaries were transferred into equilibration [7.5% (EG and DMSO)] and vitrification medium [15% (EG and DMSO) and 0.5 M sucrose], then they immersed in liquid nitrogen after loading by acupuncture needle in NIV group and cooling on pre cooled steel surface in SSV group. Thawing was done in 2steps (1 and 0 M sucrose solution). Mechanically isolated preantral follicles were cultured in α-MEM supplemented with (FSH, LH, ITS, FBS) for 12 days. The expression rate of maturation genes (GDF9 and BMP15) and their receptors (BMPR2, ALK5 and ALK6) in all experimental groups were evaluated quantitatively by real time PCR after 24 hour, 6, 10 and 12 days of culture. Results: No significant difference was observed in the expression of maturation genes and their receptors between vitrification (NIV and SSV) and control groups and also between NIV and SSV groups. It must be noted that the expression patterns of mentioned genes in two vitrification groups were similar to the control one and assimilate the in vivo pattern in somehow. Conclusion: Although the cooling rate in SSV method is delayed compared to NIV method, the pattern of maturation genes expression during IVC was similar in both groups and it seems that preantral follicles compensate cryoinjuries during IVC period.
منابع مشابه
P-116: Maturation Genes Expression during In Vitro Culture of Vitrified Mouse Preantral Follicles
Background: Evaluation of maturation genes expression and their pattern during in vitro culture of vitrified preantral follicles is an essential and could promote either vitrification procedure or culture condition. Materials and Methods: Preantral follicles (with 100- 130 μm diameter) isolated mechanically from 12-14 days old NMRI mice ovaries and divided into vitrification and control groups....
متن کاملSurvival Rate of Preantral Follicles Derived From Vitrified Mouse Ovarian Tissue by Cryotop
Purpose: The aim of this study was to investigate the growth and survival rate of preantral follicles isolated from vitrified ovarian tissue by cryotop.Materials and Methods: Ovaries of 14-day-old NMRI mice were separated and divided into three groups fowling as: vitrified, toxicity tested and control groups. Ovaries in the experimental groups were immersed in equilibration and vitrification so...
متن کاملP-83: Development of Mouse Preantral Follicles in Fibrin-Alginate Matrix during In Vitro Culture
Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...
متن کاملP-107: Vitrification and In Vitro Culture of Mouse Preantral Follicles in Presence of Growth Factors
Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...
متن کاملP-88: Expression Pattern of Maturation Genes During In Vitro Culture of Alginate Encapsulated Preantral Follicles Derived From Frozen-Thawed Mouse Ovaries
Background: This study was set up to evaluate the effect of ovarian tissue slow freezing on in vitro growth and pattern of maturation genes expression in mouse preantral follicles encapsulated within alginate hydrogel. Materials and Methods: Ovaries of 12-14 days old female NMRI mice were randomly allocated into control and slow freezing groups. In slow freezing group, ovaries were equilibrated...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 6 شماره 2
صفحات -
تاریخ انتشار 2012-09-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023