P-217: The Effect of Progesterone on The In Vitro Maturation of Mouse Germinal Vesicle Oocytes Derived from Polycystic Ovary

Background: Polycystic ovary syndrome (PCOS) is characterized by chronic anovulation and hyperandro genism and affects approximately 5-10% of women of reproductive age. In vitro maturation (IVM) of mammalian oocytes has become an efficient method to produce mature oocytes in order to use in assisted reproductive techniques such as in vitro fertilization (IVF), intra cytoplasmic sperm injection (ICSI), and cloning. In some mammals, oocytes maturation and its progression to metaphase II are steroid–dependent. Progesterone exists in the follicular fluid that contributes to normal mammalian ovarian function. It has been shown that, in vivo levels of progesterone in follicular fluid are closely associated with developmental competence of an oocyte. In PCOS, progesterone secretion and levels in ovary and follicular fluid is low. In this study, we examined the effects of progesterone on in vitro maturation of oocytes derived frompolycystic ovary in mouse. Materials and Methods: To induce polycystic ovary phenotype, female NMRI mice 6-8 weeks-old were injected daily with testosterone enantate (TE)1 mg/100 g body weight dissolved in sesame oil (experiment group), while control group were injected only with vehicle for two weeks.Collected immature oocytes (germinal vesicle oocytes) of both groups were matured in modified medium α-MEM containing combinations of 5% FBS, 100 mIU/ml rFSH (Gonal-f), 10 ng/ml rEGF, 7.5 IU/ml HCG with progesterone (3 mg/ml), (treatment I) and without progesterone (treatment II) for 24 hours. The number of oocytes at the germinal vesicle (GV), germinal vesicle breakdown (GVBD) and metaphase II (MII) stages were counted. Results: After 24 hours of transfer to maturation media, the percentage of MII oocytes in experiment group,treatments I and II, was 49.65% and 19.40% and in control group, treatments I and II was 31.51% and 36.21%. After 24 hours, exposure of progesterone, the percentage of MII oocytes were significantly increased in experiment group compared to control group (ANOVA, p<0.05). Conclusion: Adding progesterone to the medium could improve maturation of mouse germinal vesicle oocytes. It be concluded that progesterone can be to improve oocyte in vitro maturation in polycystic ovaries.