P-182: The Role of Vascular Endothelial Growth Factor Gene Expression in Patients with the History of Endometriosis

Background: Endometriosis is the presence of endometrium- like tissue in sites outside the uterine cavity, primarily on the pelvic peritoneum and ovaries. Ectopic endometrium for replacement and growth require to blood supply. Vascular endothelial growth factor (VEGF) is one of the most important intermediate of locality angiogenesis that product by monocytes and macrophages. This study evaluates gene expression of VEGF in patients with a history of endometriosis. Materials and Methods: This study contains 3 groups (n=5). In patient with endometriosis, ectopic biopsies were obtained by laparoscopic procedure and eutopic biopsies were obtain by pipelle. In women with no sign of endometriosis, control biopsies gained with piplle. Gene expression of VEGF was determined by RT-PCR and the quantitative level of gene expression was tested by real time PCR. Results: VEGF gene was expressed in all groups. However, Q-PCR analysis showed that the expression of VEGF in ectopic group was higher in compare to control and eutopic groups. Conclusion: VEGF is a potent angiogenic factor involved in physiology and pathology of angiogenesis. Elevated levels of VEGF are found in peritoneal fluid of patients with endometriosis. Peritoneal fluid macrophages elevate levels of VEGF by expressed both VEGF receptors.