P-181: The Effect of Estradiol Valerate and rFSH Combination As Ovarian Stimulator on In Vitro Fertilization and Embryo Developmental Rate of Ovulated Oocytes in Mice

Background: Sex steriods play important roles in the growth and differentiation of reproduction tissues and in maintatinance of pregnancy. 17-β oestradiol (E2) is the most important one of them that has a positive effect an the granulosa cells proliferation and ovarian follicle maturation. Then the aim of this study was determine of the effectes of rFSH+ E2 combination as an ovarian stimulator on the quality of MΙΙ oocyte and post -IVF embryos. Materials and Methods: 6-8 weeks NMRI female mice were injected intraperitonealy 10 IU rFSH and 26 μg Estradiol valerate per mouse . After 48 hours, they received 10 IU hCG as same manner and then ovulated oocytes collected from dilated part of uterine tube 18 hours after latter injection for in vitro fertilization of in T6+ 15 mg/ ml BSA. Post 6-hour fertilizated ocytes (2PNS) transfer to droplts containing T6+ 5 mg/ml BSA for 120 hours. The data were analyzed by the independent sample ttest that was two-tailed, with a confidence level of 95% (p<0.05). Results: The mean number of MΙΙ oocyte were 19.11 ± 3.79 and 14.47 ± 4.7 in control (rFSH) and experimental groups (rFSH + E2) respectively with significant difference (p=0.001) but mean of fertilization rate had no statistically difference. The mean of 2-cell embryo is better in experimental group than control. However , there were no statistically singnificant differences between the two groups in the 2PN , 4 cell, and 8-cell,embryo rates but the embryos of control group could reach to blastocycte stage. Conclusion: In this study ,we concluded that rFSH itself can cause to produce more in MΙΙ oocyte than rFSH+ E2 combination as ovarian stimulator and also Supports embryo development to blastocycte stage.