P-181: Protective Role of Vitamin E As An Alternative Treatment for Ovariectomized Osteoporotic Rats

Background: Osteoporosis one of the postmenopausal symptoms is characterized by bone loss. There is a link between excessive reactive oxygen species (ROS) formation, estrogen deficiency due to cessation of ovarian function and bone loss. Free radicals are responsible for causing osteoblast apoptosis and reducing osteoblastogenesis in bone remodeling. Vitamin E is a potent antioxidant with the ability to protect bone cells from damage by neutralizing free radicals .Its role to Scavenge ROS including in the process of osteoporosis is important. The present study was carried out in search for an alternative treatment of osteoporosis using vitamin E. Materials and Methods: The ovariectomized rat model was used in this study. Thirty mature female Wistar rats weighing approximately 200 g were selected and randomly divided into ovariectomized control, and ovariectomized rats treated with vitamin E. in experimental group treated daily at the dose of 80 IU per kg dietary. It was administrated for two months. Then the femur bone of the animals was collected and tissue bone investigated under a light and electron microscopic level. Results: The ovariectomized rats showed a significant decrease in bone mass density of femur in control group with an increase in resorption and reduction in both trabecular volume and number. Bone loss induced deterioration of bone trabecula in comparison with normal tissue bone they showed inappropriate lamellar structure and a large uncalcified bone matrix. In experimental group vitamin E prevented the reduction in trabecular bone content and decreased trabecular separation bone. Administration of vitamin E reversed bone loss and prevented destruction of bone tissue and trabecular formation. Serum biochemical assays revealed that vitamin E. prevent osteoporosis induced in ovariectomized group (p<0.05). Conclusion: In this study, we investigated the effects of vitamin E which act against of bone degeneration and alternation in bone after osteoporosis. It is suggested that vitamin E has the potential effect as an alternative and effective treatment for prevention of bone loss in postmenopausal osteoporosis especially order with other routine treatment methods.