P-172: Intracellular Toll Like Receptors Expression in Endometriosis

Background: Endometriosis is a complex disease that profoundly affects the quality of life in many women. This disease affects roughly one in ten women of reproductive age. Endometriosis induces a variable amount of inflammatory reaction in pelvic environment. An active immune system needs to recognize these inflammatory agents. Rapid innate immune system defenses against infections involve the recognition of invading viral and bacterial pathogens, by the family of Toll like receptors (TLRs). Among TLRs family only TLR3, 7, 8 and 9 that expressed in the intracellular endosomal compartments, which can detect viral infections.TLR3 distinguishes double strand RNA viral motifs. TLR 7/8 are specific for single strand RNA. While TLR9 recognizes unmethylated CPG DNA of viruses. The objective of this study is to clarify the expression of intracellular TLRs in the woman with endometriosis. Materials and Methods: In this study three groups were examined. Ectopic biopsies were obtained with laparoscopic procedure from patient with endometriosis. Eutopic and control biopsies were gained with piplle from endometrium of women with and without endometriosis. Reverse transcriptase polymerase chain reaction (RTPCR) and quantitative-PCR (Q-PCR) for 5 samples of each groups used to show the existence of TLR3, 7, 8 and 9 genes. Results: TLR3, 7, 8 and 9 mRNA were expressed in the each groups but in ectopic and eutopic samples we showed variable expression. Conclusion: Our finding suggested that TLRs is involved in endometriosis pathophysiology. It is shown that some products of TLRs signaling such as TNf-α and IL-1 were increased in endometriosis.