P-15: Effects of Vitamin E and Cystein on Semen Quality and Biochemical Parameters Related with Lead Toxicity in the Male Rat

نویسندگان

  • Asadpour R
  • Kianifard D
  • Tayefi H
  • Zaboli N
چکیده مقاله:

Background: This study was undertaken to investigate whether treatment with vitamin E and N acetyl cystein protects rat sperm by inhibiting reactive oxygen species generation induced by lead (Pb) exposure. Materials and Methods: After an acclimatization period of one week, 20 Male rats were divided into 4 groups of 5 rats each. Animals within different treatment groups were maintained on their respective diets for 6 weeks as follows: group 1, untreated control; group2, treated orally with lead acetate (1000 ppm); group 3, treated orally with vitamin E (20 ppm) plus lead acetate (1000 ppm); group4 treated orally N-acetyl cystein (800 ppm) plus lead acetate (1000 ppm). The blood and sperm Pb levels were analyzed by graphite furnace atomic absorption spectrophotometer. Results: Significant decrease in the weights of testes and epididymis were observed in lead treated animals. Exposure to lead acetate significantly increased malondialdehyde levels with a significant decrease in the superoxide dismutase and catalase activities in testes of rats. Epididymal sperm count, viable sperms, motile sperms decreased significantly in lead-exposed rats. Co-administration of vitamin E and N acetyl cystein to lead exposed rats showed a significant increase in the weights of reproductive organs, reduction in leadinduced oxidative stress in the testis tissue and improvement in selected reproductive parameters.Conclusion: The rats received vitamin E + Pb and cystein + Pb could enhance antioxidant system and semen quality which consequently reduced the oxidative strees and improvement reproduction in rat.

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عنوان ژورنال

دوره 6  شماره 2

صفحات  -

تاریخ انتشار 2012-09-01

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