P-123: Impact of Oxidative Stress on Differentiation of Testis in Mice

Background: Antioxidants and reactive oxygen species (ROS) are in balance in the body. Whenever the balance between these molecules is disrupted towards an overabundance of ROS, oxidative stress (OS) occurs. There are evidence that glutathione as an intracellular antioxidant plays an important role against ROS in female reproductive system. Regarding the effect of BSO as a selective inhibitor of glutathione peroxides. On the other hand, primary germ cells after migration to developing ovary differentiate to oogonia and oocytes. Formation and number of primordial follicles in early fetal life is a determining factor in the fertility state of adult life .The aim of the present study is to investigate BSO induced OS on development and differentiation of ovarian follicles in mice. Materials and Methods: In this study 30 adult female and 15 adult male mice are used. The female mice are divided into two groups of control and experimental .Two female mice at their stereos cycle were put with one male mouse in a cage for mating. Observation of vaginal plaque was considered as the first day of pregnancy and the mice on 13th day of pregnancy received 2 mmol/kg BSO daily until delivery as IP injection. After the pregnancy the 2, 3, days old new born were sacrificed and their testis were fixed and prepared for light and electron microscopic studies. In the sections, the number seminiferous tubules and diameter of them were determined using Motic software and compared with control values using t-test. Results: Light and electron microscopy and morphometry showed that seminiferous tubules are distinguished on the 2nd and 3rd day’s old newborn. Morphometric studies revealed that the number and diameters of seminiferous tubules were significantly (p<0.001) reduced in Experimental groups in comparison to control group. Conclusion: The result indicates that oxidative stress suppresses follicular differentiation at early stages but does not affect the development of already differentiates follicles.