P-12: Histological and Histometrical Studies of Rat Prostate Lobes Following Administration of Testosterone

growth of the prostate gland, also development of benign prostatic hyperplasia (BPH) is an androgen-dependent functions. Testosterone plays a major role in the prostate gland. Rat prostate gland is one of the most widely used models of animals in investigation. The rat prostate gland consisted of five lobes; the ventral, anterior, dorsal, lateral type 1 and latteral type 2. The goal of present study was to reveal the influence of testosterone on histomorphological structure of different lobes of rat prostate gland. Materials and Methods: For this purpose, 15 adult and healthy male rats in 3.5-4 months of age and weighing 250-300 g were selected and divided in three groups 1: control group, group2, received solvent of testosterone (almond oil) 10 mg/kg/day(SC), group3, received testosterone (SC) 10 mg/kg/day. The rats were kept in 12L 12D and 25°C temperature for 6 weeks. Then, rats were sacrificed, weighted and prostate glands were removed. The weight and volume of prostate were measured and fixed in buffer formalin 10%. Samples were taken from different lobes. The 5-6 μ sections were made using paraffin embedding method and were stained with H&E and PAS staining. The histometrical studies were done using Dino digital Lens and Dino soft ware capture1. Results: The results showed that prostate/rat weight and prostate volume in the testosterone group were increased significantly compared to the other groups (p <0.0001). Micrometry results indicated that the maximum effect of testosterone on the prostate gland were seen in ventral and anterior lobes.The parenchyma / stroma ratio (p<0.05), thickness of the epithelium (p <0.0001), lumen diameter of secretary units and number of secretory cells (p <0.05) were increased significantly in the ventral lobe of group received testosterone. Conclusion: The results showed that different lobes have different response to T and ventral and anterior lobes are more sensitive to administration testosterone, which it must be consider in rat prostate investigation.