P-116: The Catalase Effect on LDH Enzyme Leakage of Buffalo Epididymal Sperm During Culture in Human Tubal Fluid Mediaa

Background: Mammalian spermatozoa are highly susceptible to lipid peroxidation, which leads to structural damage to the sperm cell, the enzymatic leakage from spermatozoa accompanied by lowered motility and metabolism. Catalase, an enzyme that detoxify hydrogen peroxide and reduce the enzyme leakage from sperm cell Materials and Methods: For this study, buffalo bull testicles (20 pairs) were picked up in the march till September 2013 in Urmia local slaughterhouse and transported to the laboratory in a cool container (filled with 5°C ice pack). Epididymis were dissected and cleaned from connective tissue. Sperm were collected performing several incisions in the cauda epididymis with a surgical blade, and taking the white fluid emerging from the cut tubules. Five levels of catalase (50-100-200-400-800 IU) were added into human tubal fluid media (HTF) containing sperms (30×106 sperm/ml), with 10%, bovine serum albumin and were incubated for 36 hours at 37 ºC.Sperm LDH enzyme assay was performed at 1-4-12- 24 and 36 hours based on “Deutsche Gesellschaft Fur Klinische Chemie (DGKC)” protocol. After collection of data, Statistical analyses were performed with procedures available in ANOVA of SPSS version 20. Results: The results showed that, adding catalase enzyme on buffalo epididymal sperm cells increase LDH enzyme leakage. Conclusion: Catalase has no effect on sperm cell membrane integrity during culture in HTF media.