Optimization of copper nanoparticles synthesis using E. coli and the study of its antifungal activity

Objective (s): The green synthesis of copper nanoparticles using metabolites of microorganisms has gained much interest in recent years. In this work, it was studied optimization of copper nanoparticles synthesis using E. coli and its antifungal activity.Materials and Methods: The copper nanoparticles were synthesized by Escherichia coli. Effect of copper nitrate concentration and temperature was studied on size and production efficiency. In addition, copper nanoparticles were analyzed by UV – VIS spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS). Finally, the antifungal properties of synthesized nanoparticles were tested against Penicillium by disc diffusion method in different concentrations of nanoparticles.Results: It was found that initial concentration of copper nitrate plays a key role in formation of nanoparticles. Also, it was indicated that in lower temperatures, the size of copper nanoparticles is smaller and their distribution is narrower. It was determined the concentration of 15% w/v of copper nanoparticles in distilled water is optimum concentration for the maximum of antifungal activity. Conclusion: The biosynthesized copper nanoparticles displayed antifungal activity against Penicillium. The experiments showed the usability of these nanoparticles in water purification, air purification and antifungal packaging.