Molecular characterization of recent Iranian infectious bronchitis virus isolates based on S2 protein gene
نویسندگان
چکیده مقاله:
Background: Avian infectious bronchitis (IB), with avianinfectious bronchitis virus (IBV) as the causing agent, is a ubiquitous endemicdisease of the chicken with devastating effects on its industry. A viralmembrane surface protein called S notonly induces neutralizing antibodies but also plays an important role in virusbinding and entry to host cells. Technically, S1 protein gene sequencing alsohelps greatly in IBV genotyping. OBJECTIVES: The aim of this study wasto characterize Iranian IBV based on S2 gene. METHODS: After RT-PCRamplification, the S2 gene of nine Iranian IBV isolates were sequenced and thencompared with reference strains. RESULTS: The isolates were classifiedinto genotype I as Massachusetts like IB Vs, genotype VII which clustered intotwo branches, VIIa (IS-1494 like IB viruses), and VIIb, and was related to QX-like viruses and Genotype VIII as 793/B like IBVs. CONCLUSIONS: As faras we know, this is the first S2-based classification study on Iranian IBVisolates providing a firm experimental basis to correlate with genotypiccharacterization
منابع مشابه
molecular characterization of recent iranian infectious bronchitis virus isolates based on s2 protein gene
background: avian infectious bronchitis (ib), with avianinfectious bronchitis virus (ibv) as the causing agent, is a ubiquitous endemicdisease of the chicken with devastating effects on its industry. a viralmembrane surface protein called s notonly induces neutralizing antibodies but also plays an important role in virusbinding and entry to host cells. technically, s1 protein gene sequencing a...
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عنوان ژورنال
دوره 10 شماره 4
صفحات 245- 251
تاریخ انتشار 2016-12-01
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