Investigation of VPR2 gene expression in AGS cells transfected with recombinant vector carrier of tagD gene of Helicobacter pyloriExpression of VPR2 Gene in AGS

Backgrounds: Helicobacter pylori is associated with the development of gastric cancer. The thiol peroxidase enzyme, encoded by the tagD gene in this bacterium, plays an important role in bacterial attachment and colonization in the human stomach. The aim of this study was to investigate the expression of VPR2 gene in AGS cells transfected with recombinant vector of helicobacter pylori tagD gene. Materials and methods: In this study, AGS cells were cultured in RPMI-1640 medium containing 10% bovine serum and in standard conditions. These cells were transfected with the recombinant vector PFLAG-CMV-3-tagD or the empty vector PFLAG-CMV-3 as a control. Whole cell RNA was extracted and cDNA synthesized. Real-time RT-PCR was then performed to evaluate the expression of VPR2 gene in both transfected and control AGS cells. Finally, expression of each gene was evaluated using SPSS software and t-test Indepent. Results : Eukaryotic expression of tagD gene in AGS cells of tagD gene was confirmed by RT-PCR. Expression of VPR2 genes in AGS cells receiving tagD gene was significantly higher than control cells (p = 0. 0203). Conclusion: Based on the findings, it can be concluded that the helicobacter pylori tagD gene, by affecting AGS cells, can decrease or increase gene expression in these cells. The tagD gene is one of the most important Helicobacter pylori genes that can affect host cells.