Investigation of the prevalence of Campylobacter species and their antibiotic resistance phenotypes among poultry meat samples in 22 regions of Tehran, Iran

نویسندگان

  • Ahmadi, Fatemeh Department of Microbiology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
  • Alebouyeh, Masoud Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Besharati, Saeed Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran
  • Eslami, Parisa Pathology Laboratory, Mehr General Hospital, Tehran, Iran
  • Fani, Fereshteh Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Farzami, Marjan Rahnamaye Health Reference Laboratory Research Center, Reference Health Laboratory, Ministry of Health and Medical Education, Iran
  • Ganji, Leila Health Reference Laboratory Research Center, Reference Health Laboratory, Ministry of Health and Medical Education, Iran
  • Majidpour, Ali Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
  • Moghadam, Somayeh Soleymanzadeh Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
  • MohammadSalehi, Reza Molecular Microbiology Research Center, Faculty of Medicine, Shahed University, Tehran, Iran
  • Nikmanesh, Bahram Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
  • Owlia, Parviz Molecular Microbiology Research Center, Faculty of Medicine, Shahed University, Tehran, Iran
  • Pouladfar, Gholamreza Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Sadeghi, Atena Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran
  • Tajeddin, Elahe National Nutrition and Food Technology Research institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده مقاله:

Background and Objective: Pathogenic species of Campylobacter, in addition to diarrhea and gastrointestinal diseases, could cause debilitating auto-immune and chronic diseases in humans. Investigation of the existence of this bacterium in food sources and clinical samples, and detection of antibiotic resistance could be helpful in the control of its spread and treatment procedures. The aim of this study was to evaluate the frequency and antibiotic resistance of Campylobacter isolates isolated from poultry meat. Materials and Methods: In this study, a total of 100 poultry meat samples were collected from 22 districts of Tehran from July 2018 until March 2019. Accordingly, standard enrichment of the collected samples and their culture in selective medium was done. The isolates were characterized biochemically and by polymerase chain reaction for genus and species specific primers for C. jejuni, C. coli, C. upsaliensis, and C. lari. Antimicrobial susceptibility of the isolates to 7 antibiotics were done using E-test and disk diffusion methods. Moreover, resistance to three or greater classes of antibiotics was determined as multiple drug resistance phenotype (MDR) and the results were statistically analyzed using SPSS16 software. Results: Campylobacter was isolated from 35% of the poultry meat samples. C. jejuni (23%), C. coli (1%), and C. lari (1%) were among the Campylobacter isolates from these samples. Highest resistance phenotype and the lowest ones were detected against tetracycline (62.8%), and ampicillin and clindamycin (17.1%, each one), respectively. The MDR phenotype was detected among 42.8% of the isolates. Conclusion: Our results showed high level of contamination with Campylobacter in the poultry meat samples which proposed increased risk of the infection with MDR strains among the consumers in Tehran. Further studies on human clinical samples could better determine this correlation.

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عنوان ژورنال

دوره 27  شماره None

صفحات  1- 8

تاریخ انتشار 2020-02

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