Investigation of the effect of experimental polycystic ovarian syndrome induced by stradiolvalerate on oocyte quality and in vitro fertilization potential and evaluation of vitamin E supplementation to emryo culture in mouse model

Background & Aims: One of the most common causes of infertility in women is ovarian causes, especially polycystic ovary syndrome (PCOS). However, in patient with PCOS, the number of oocytes taken increased during the IVF, but these oocytes have often low quality, which ultimately leads to reduce fertilization potential. The aim of this research was investigation of PCOS on oocytes quality and potential of IVF and and evaluation of the effect of vitamin E supplementation to embryonic culture medium, as well. Materials & Methods: In this experimental syudy100 female mice were divided into 2 groups: PCOS and control. To create an experimental PCOS stradiolvalerate injected itraperitonealy (100mg/kg). After 8 weeks IVF process was performed and the quality of oocytes, fertilization rate and embryo development process were evaluated. Finally, the effect of vitamin E with doses of 100, 200, 400 micromol, (p<0.05) were investigated in embryo culture. Results: Comparison of results showed that experimental PCOS group showed a significant decreasein oocyte quality and fertilization, dualcell, blastocyst and significant increase in lysis and fragmentation in stopped embryos, on the other hand lysis and fragmentation, reduction of percentage and type of stopped embryos in presence of 100 and 200 micromole vitamin E concentrations were significantly in compare with PCOS group (p<0.05). Discussion & Conclusion: Finally it can be concluded that experimental PCOS decreases the quality of oocytes and IVF and the addition of vitamin E to fetal culture medium as antioxidant can improve fertility.