In vitro Comparison of Viability of Human Gingival Fibroblast Cells on Collagen Barriers

 Background and purpose: Human gingival fibroblasts cultured on collagen membrane is an alternative treatment method in guided regeneration. This in vitro study aimed at evaluating and comparing the human gingival fibroblasts viability on two types of collagen-rich membranes. Materials and methods: Human gingival fibroblast cells (HGF1-RT1) were cultured on two types of collagen-rich membranes, including Regen and CenoMembrane. After 24 and 72 hours, the MTT test was performed. ANOVA test and Post Hoc Tukey were applied to compare MTT mean values in different groups and times. Results: After 24 and 72 hours, the highest rates of viability of fibroblast cells were observed in the control group and the Regen membrane, and the lowest viability was seen in the CenoMembrane group. Cell viability in control group was significantly higher than the CenoMembrane and in Regan membrane was significantly higher than the CenoMembrane (P<0.05). There were no significant differences between the Regen membrane and the control group (P>0.05). Conclusion: Based on MTT assay, the viability of fibroblasts after 24 and 72 hours was higher in the Regen membrane compared with the CenoMembrane.