Examination of the Effect of Application Time of Titanium and Methyl Jasmonate Nanoparticles on Physiological, Growth, and Biochemical Characteristics of Savory Daenesis (Satureja hortensis L)

An experiment was performed to examine the effect of methyl jasmonate foliar spray with concentrations (10, 1, 0.1 mM and 0) and titanium nanoparticles with concentrations (40, 30, 20  µM and 0) on the physiological indices of Savory. The field experiment was carried out in Isfahan in the summer of 2019. This experiment was conducted in a factorial manner based on completely randomized design and 3 replications with 2 factors and 4 levels in two harvest times of 24 and 48 hours after the last treatment .According to the results of this research, compared to the control treatment, methyl jasmonate treatments result in a significant increase of 1 percent in leaf area, leaf fresh weight, leaf dry weight and length of aerial part in 48 hours after the last treatment. Titanium nanoparticle treatments result in a significant increase at the level of 1 percent in leaf width, leaf area, leaf dry weight, leaf fresh weight and length of the aerial part of the plant. Similarly, the flavonoid content increased significantly at a concentration of 30 μM at the level of 1 percent. In combined treatments that were conducted at 48 hours after the last treatment, there was a significant increase in leaf dry weight, leaf area, leaf fresh weight and length of aerial part at the level of 1 percent and a significant increase at the 5 percent level in flavonoid content and activity of dismutase enzyme. It was concluded from the current research that 48 hours after the last treatment is the best time for harvesting. The use of both concentrations of 1 or 0.1 mM methyl jasmonate with 40 μM nanoparticles and 10 mM methyl jasmonate with 30 μM nanoparticles affects the plant positively and can increase the growth and biochemical factors of Savory.