Evaluation the effect of analog curcumin on the display and expression of SIRT1 and FAS genes in HepG2 fatty cells.

Abstract: Background: Non-alcoholic fatty liver is a disease that will lead to liver cirrhosis if not treated. Curcumin is the active substance of the rhizome of the turmeric plant, which has antioxidant, anti-inflammatory, antimicrobial, etc. properties. In the present study, the effects of curcumin analogue on the expression of SIRT1 and FAS genes and the accumulation of triglycerides in fatty HepG2 cells (fatty liver model) have been investigated.  Materials and methods: After cultivating HepG2 cells in suitable environment and conditions, MTT test was used to select the appropriate dose for curcumin and its analogue. After oiling the cells and treating them with the desired compounds, RNA was extracted and cDNA was made, then real-time PCR was used to investigate the effect of curcumin and its analog on the expression of SIRT1 and FAS genes. Results: As a result of the treatment of fatty HepG2 cells (fatty liver model) with semi-synthetic derivative of curcumin, the relative expression of SIRT1 gene and SIRT1 enzyme activity increased significantly compared to the control group. On the other hand, the relative expression of FAS gene compared to the control group, as well as the accumulated triglycerides in these cells has decreased significantly. Conclusion: The results show that the treatment of HepG2 fatty cells (fatty liver model) with curcumin and its derivative significantly increases the relative expression of SIRT1 gene and its enzyme activity, as well as significantly decreases the relative expression of FAS gene and accumulated triglycerides in fatty cells. HepG2 (fatty liver model) compared to the control group.