Evaluation of effect of chick embryonic notochord on neural induction of mouse embryonic stem cells

evaluation of effect of chick embryonic notochord on neural induction of mouse embryonic stem cells

introduction: the aim of this study was evaluate the ability of notochord to induce neural induction and/or differentiation of mouse embryonic stem cell to neuron and motor neuron, respectively. methods: in order to produce embryoid bodies, es cells line royan b1 were grown in suspension in the absence of lif for 4 days. ebs were divided into 4 groups. ebs in group 1 & 2 were further cultured i...

The Effect of chick Embryonic Somites on Neural Rosette Formation in Mouse Embryonic Stem Cells

purpose: The aim of the present study is to understand if EBs can generate neural rosette upon co-culture with chick embryo somites. Materials and Methods: The mouse ES cells, line Royan Bl, were cultured in hanging drops to induce embryoid bodies (EBs) formation. Somites were isolated from the chick embryos and then embedded in alginate solution. Finally, alginate beads containing somites were...

Differentiation of Mouse Embryonic Stem Cells into Hematopoietic Cells

Purpose: Differentiation of Mouse embryonicstem cells into Hematopoietic cells. Materials and Methods: In this study, we used EB formation system for Hematopoietic differentiation of mouse embryonic stem cell (Royan B1) in suspension culture. EBs cultured in medium with Hematopoietic inducer cytokines (SCF, TPO, GMCSF, IL3, Flt3 and EPO) .presence of hematopoietic differentiated cell assessed ...

The Effect of Astrocyte-Conditioned Medium (ACM) and Retinoic Acid on Neural Differentiation of Mouse Embryonic Stem Cells

Purpose: The aim of this research was to study the properties of factors secreted from astrocyte cells in suspension medium in direct differentiation of mouse embryonic stem cells into neural cells. Materials and Methods: Royan B1 mouse embryonic stem (ES) cells were used in this experiment. For differentiation of Es cells into the neural cells, the astrocyte-condition medium (ACM) of mouse fe...

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells

the effect of mouse embryonic fibroblast in direct differentiation of mouse embryonic stem cells

background: since embryonic stem (es) cells have the dual ability to proliferate indefinitely and differentiate into multiple tissue types, es cells could potentially provide an unlimited cell supply for human transplantation. objective: in order to study the differentiation of mouse embryonic stem (mes) cells, they were cultured in suspension by using es media without leukemia inhibitory facto...