Evaluation of Chronotropic Properties of Mouse Embryonic Stem Cells-Derived Cardiomyocytes After Fibroblast Growth Factor Treatment
نویسندگان
چکیده مقاله:
Purpose: We investigated the effect of (bFGF) (basic-Fibroblast Growth Factor) on the differentiation of divided cardiomyocytes from mouse embryonic stem cells (ES) and their pharmacological properties. Materials and Methods: The mouse embryonic stem cells (Royan B1) were cultured as 800 cells per 20µl of a hanging drop. After two days, ES cells in each drop aggregated to form embryoid bodies (EBs). The EBs were cultured in bacterial dishes for 5 days. In the first two days of culture in the bacterial dishes, the cells were treated with 10ng/ml bFGF.Then the EBs were plated in gelatin- coated 24-well plates individually, and the beating rates of the EBs were recorded in the control and bFGF groups no a daily basis.Moreover, the chronotropic effects of isoprenaline, carbachol and phenylephrine on ES cells-derived cardiomyocytes were investigated. Results: Treatment of cardiomyocytes with isoprenaline and phenylephrine, increased the rate of heart beat in both groups, but this rate was enhanced in early stages of development in the bFGF group more than that in the controls (p<0.035 and p<0.015). Furthermore, although, carbachol decreased the heart rate, this difference was not statistically different in both groups. The beating rate was higher in the EBs in the control group than that in the bFGF group (7+5 day with p<0.012, 7+7 day with p<0.002, 7+9 day with p<0.0001, 7+17 day with p<0.014, 7+19 day with p<.001 was significant). Also, the beating rate was found to the more stable in the control group. Conclusion: These results showed that although bFGF probably exerts its effects on the expression or function of some cardiomycytes receptors, individually it has no significant effect on cardiogenesis.
منابع مشابه
the effect of basic fibroblast growth factor on differentiation of mouse embryonic stem cells into cardiomyocytes
introduction: embryonic stem (es) cells are pluripotent cells that derived from inner cell mass (icm) of blastocyst stage and are able to differentiate in vitro into different cells. purpose: this study was initiated to evaluate the effect of basic fibroblast growth factor (bfgf) on differentiation of es cells into cardiomyocytes among with their response to cardiac drugs. methods: the mouse em...
متن کاملThe Effect of Cardio Gel and Matrigel on the Ultrastructure of Cardiomyocytes Derived From Mouse Embryonic Stem Cells
Purpose: To investigate the effect of cardiogel and matrigel on the ultrastructure of embryonic stem cell-derived cardiomyocytes. ECM: Extracellular Matrix derived from cardiac fibroblasts (cardiogel), commercial extracellular matrix (matrigel) and control group (without ECM) were cultured for up to 21 days. Ultrastructural properties of cardiomyocytes were evaluated by transmitting electron mi...
متن کاملThe effect of Fibroblast Growth Factor-2(FGF-2) and retinoic acid on differentiation of mouse embryonic stem cells into neural cells
Introduction: Embryonic Stem (ES) cells as pluripotent cells derived from the inner cell mass of blastula can differentiate to neural cells in vitro and this property is valuable in studies of neurogenesis and in the generation of donor cells for transplantation. In this regard, the propose of this research, was the study of the role of two important factors in the development of neural syst...
متن کاملkinetic properties of lactate dehydrogenase extraeted from mouse embryonic stem cell-derived cardiomyocytes and mouse neonatal cardiomyocytes: a comparative study
introduction: lactate dehydrogenase is one of the cardiac enzyme markers, studied in vivo. in the present study, activity and kinetic properties of enzyme extracted from cardiomycytes derived from mouse embyronic stem cell in vitro were compared with enzyme extracted mouse neonatal from cardiomyocytes in vivo. material and methods: mouse embryonic stem cells were cultured and differentiated and...
متن کاملImprovement of Expression of α6 and β1 Integrins by the Co-culture of Adult Mouse Spermatogonial Stem Cells with SIM Mouse Embryonic Fibroblast Cells (STO) and Growth Factors
متن کامل
Evaluation of effect of chick embryonic notochord on neural induction of mouse embryonic stem cells
Introduction: The aim of this study was evaluate the ability of notochord to induce neural induction and/or differentiation of mouse embryonic stem cell to neuron and motor neuron, respectively. Methods: In order to produce embryoid bodies, ES cells line Royan B1 were grown in suspension in the absence of LIF for 4 days. EBs were divided into 4 groups. EBs in group 1 & 2 were further cultur...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 2 شماره 1
صفحات 11- 17
تاریخ انتشار 2004-04
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
کلمات کلیدی برای این مقاله ارائه نشده است
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023