Evaluation of antibacterial effect of propolis nanoemulsion, Ethanolic Extract of propolis, Ciprofloxacin and usage of their combination against Pseudomonas aeruginosa PAO1

Background: Pseudomonas aeruginosa is one of the most important opportunistic pathogens and is considered as one of the main causes of nosocomial infections. Due to the increasing antibiotic resistant and the risk of antibiotic resistance, studies on the identification of natural antibacterial compounds have increased. In this study Antibacterial properties of Ethanolic extract of propolis, propolis nanoemulsion, Ciprofloxacin and their combination on Pseudomonas aeruginosa PAO1strain  have been studied. Materials and Methods: In this study, high-energy propellant nanoemulsion was prepared using ultrasound waves. Antibacterial effect of ethanolic extract of propolis, propolis nanoemulsion, Ciprofloxacin alone and their combinations (EEP+Ciprofloxacin and propolis nanoemultion + ciprofloxacin), were determined. For determination of MIC, MBC and FIC of each substance, Broth microdilution and disk diffusion test were used. Data Statistical analysis was performed using SPSS software and one way ANOVA test. Results: The results show that the Minimum inhibitory concentration of Ethanolic Extract of Propolis and propolis nanoemulsion against pseudomonas aerouinosa is 1000 and 468.8 μg /ml, respectively. Also, the evaluation of the combined effect of Ethanolic Extract of propolis and propolis and propolis nanoemulsion with ciprofloxacin were 9.76/2.44,7.3/1.22 μg /ml respectively. The diameter of Growth inhibition zone of combination propolis nanoemulsion with Ciproflioxacin 7.3/1.22 μg /ml and propolis nanoemulsion alone 468.8 μg /ml were 15 Millimeter.   Conclusion : The propolis nanoemulsion alone and accompanied with ciprofloxacin have a high antimicrobial and inhibitory effects on Pseudomonas aeruginosa. Also, the combination of propolis nanoemulsion with ciprofloxacin has a synergistic effect.