Evaluation of Acid Phosphatase Activity Alterations in Mouse Endometrium After Ovarian Hyperstimulation During Early Natural and Pseudo Pregnancy Until Implantation Time

Purpose: The purpose of this study was to determine acid phosphatase (ACP) activity of uterus in natural and pseudo pregnanat mice after ovulation stimulation usmg PMSG and hCG during preimplantation period. Materials and Methods: In this experimental research, 6-10 week old female NMRI mice were selected and divided into two groups: control and hyperstimulated. These mice were rendered normal pregnancy or pseudopregnancy using vaginal stimulatin. Five mice per each group were sacrificed by cervical dislocation at the first day to sixth day of pregnancy. For biochemical assay, samples obtained from the uterine horns were hemogenated , and then centrifuged with 14000g. The activity of enzyme was determined using paranitrophenyl phosphate as substrate, then specific activity of enzyme (V/mg) was calculalated according to the total protein (mg/dl). For hisotchemistry another sampels were obtained from uterus, then the cryosections were provided with 5 mm thickness and stained with Goumorys method. Results: The result showed that the biochemical findings were correlated with the histochemical observation. The ACP activity of uterus was increased from day I to day 4 of pregnancy and after that it was decreased. Almost there was no significant difference between ACP activity of hyperstimulated and non-stimulated groups.The enzyme reaction changes were seen mainly in epithelial cells of endometrium but their activity was constant in lamina propria and myometrium. Conclusion: Thus our results showed that increase in ACP activity in day 3-4 of pregnancy, emphasises the role of ACP in implantation. Also ovulation induction, can not change the pattern of ACP activity of endometrium during early pregnancy which requires further research.