Enhanced Immune Responses of a Hepatitis C Virus core DNA Vaccine by co-Inoculating Interleukin-12 Expressing Vector in Mice

enhanced immune responses of a hepatitis c virus core dna vaccine by co-inoculating interleukin-12 expressing vector in mice

background: hepatitis c (hcv) is a worldwide problem without an effective vaccine with more than 170 million chronically infected people worldwide. dna vaccines expressing antigenic portions of the virus with adjutants have recently been developed as a novel vaccination technology. objectives: in the present study, a dna vaccine expressing hcv core protein was developed with il12 as a genetic a...

Immunogenicity Evaluation of a DNA Vaccine Expressing the Hepatitis C Virus Non-Structural Protein 2 Gene in C57BL/6 Mice

Backgrounds: Most of the hepatitis C virus (HCV) infections elicit poor immune responses and 75% to 85% of cases become chronic therefore, the development of an effective vaccine against HCV is of paramount importance. In this study, we aimed to evaluate co-administration of HCV non-Structural Protein 2 and IL-12 DNA vaccines in C57BL/6 mice. Methods: A plasmid encoding full-length HCV NS2 prot...

Immune Responses against a New HIV-1 p24-gp41/pCAGGS-IL-12 DNA Vaccine in Balb/c Mice

Background: Development of an effective vaccine is highly needed in order to restrict the AIDS pandemic. DNA vaccines initiate both arms of immunity without the potential of causing disease. HIV-1 p24 and gp41 (gag and env) proteins play important roles in viral pathogenesis and are effective candidates for immune induction and vaccine design. Objective: In this study, new DNA vaccine candidate...

ubiquitin conjugation of hepatitis b virus core antigen dna vaccine leads to enhanced cell-mediated immune response in balb/c mice

Assessment of humoral immune response of a Cytomegalovirus DNA-vaccine candidate in BALB/c mice

Introduction: Glycoprotein B (gB) is the major antigen for induction of humoral responses against human cytomegalovirus (HCMV) making it an attractive candidate for immune prophylaxis. In the present study, the humoral immune response of BALB/c mice to a truncated HCMV gB protein fused with GFP was evaluated. Methods: The truncated gB coding sequence was synthesized and cloned in pEGFPN1 eukary...

immune responses against a new hiv-1 p24-gp41/pcaggs-il-12 dna vaccine in balb/c mice

background: development of an effective vaccine is highly needed in order to restrict the aids pandemic. dna vaccines initiate both arms of immunity without the potential of causing disease. hiv-1 p24 and gp41 (gag and env) proteins play important roles in viral pathogenesis and are effective candidates for immune induction and vaccine design. objective: in this study, new dna vaccine candidate...